| 荧光波长对共焦显微镜成像特性的影响 |
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| 引用本文： | 裴红津,唐志列,杨初平,梁瑞生,汪洁,徐险峰.荧光波长对共焦显微镜成像特性的影响[J].光学学报,2002,22(10):219-1223. |
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| 作者姓名： | 裴红津 唐志列 杨初平 梁瑞生 汪洁 徐险峰 |
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| 作者单位： | 1. 华南师范大学物理系,广州,510631
2. 华南师范大学物理系,广州,510631;华南农业大学理学院,广州,510642 |
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| 基金项目： | 广东省自然科学基金 (980 0 4 6 )资助课题 |
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| 摘 要： | 导出了共焦显微镜中不同荧光波长情况下的荧光功率传输函数、三维脉冲响应函数（3D－PSF）和三维光学传递函数（3D－OTF）。结果表明，不同的荧光波长对共焦显微镜的空间截止频率、分辨率、光学传递函数存在明显的影响。当激发波长与荧光波长的比值下降到一定程度时，可以看到明显的失锥现象。
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| 关 键 词： | 荧光波长 共焦显微镜 成像特性 失锥现象 |
| 收稿时间： | 2001/12/10 |
Influence of Fluorescent Wavelength on Imaging Property of Confocal Microscopy |
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| Pei Hongjin Tang Zhilie Yang Chuping , Liang Ruisheng Wang Jie Xu Xianfeng.Influence of Fluorescent Wavelength on Imaging Property of Confocal Microscopy[J].Acta Optica Sinica,2002,22(10):219-1223. |
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| Authors: | Pei Hongjin Tang Zhilie Yang Chuping Liang Ruisheng Wang Jie Xu Xianfeng |
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| Institution: | Pei Hongjin 1) Tang Zhilie 1) Yang Chuping 1),2) Liang Ruisheng 1) Wang Jie 1) Xu Xianfeng 1) 1),Department of Physics,South China Normal University,Guangzhou 510631 2),College of Science,South China Agricultural University,Guangzhou 510642 |
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| Abstract: | Fluorescence power transfer function, three dimensional point spread function (3D PSF) and three dimensional optical transfer function (3D OTF) for the various fluorescent wavelength are calculated. The results show that the fluorescent wavelength has influence on imaging property of confocal microscopy such as spatial cut off frequency, resolution and 3D OTF. There is a missing cone in the 3 D space of OTF when the ratio of excitation wavelength to fluorescent wavelength decreases. |
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| Keywords: | fluorescent wavelength confocal microscopy imaging property |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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