激光高精度细胞微手术机理的研究

激光微手术的精度通常受到热作用或者热机械效应间接损伤的限制。把激光脉宽调整到与吸收颗粒的热弛豫时间一致可以避免热副作用，可对所选细胞产生选择性处理。这里胶体金与牛肠硷性磷酸脂酶（Alkaline phosphatase，AP）结合物作为分析强吸收颗粒附近的蛋白质变性的模型系统，利用皮秒和纳秒激光对结合体进行照射，在一定的条件下可以使蛋白质失活，并可以使细胞膜的通透性提高。通过计算可知，采用脉宽为皮秒或者纳秒级的激光照射纳米吸收物（胶体金）时，颗粒很容易被加热到几百开，而且热效应仅限于几十纳米的空间范围内。利用纳米吸收颗粒进行激光微手术的方法．在蛋白质失活的情况下对其机弹讲行了分析．

Study on the Fundamental of the Laser High-Precision Microsurgery

The precision of laser-microsurgery is often limited by thermal and thermomechanical collateral damages.Adjusting the pulse width of the laser to the thermal relaxation time of the absorbing construction can avoid thermal side effects and facilitate a selective treatment of the selective cells.Conjugates of colloid gold and bovine intestinal alkaline phosphatase(AP) are presented as the model system for investigating protein inactivation in the vicinity of strongly absorbing nanoparticles,and under certain conditions the protein can be denatured and the membrane permeabliltiy can be increased with the irradiation of the nanosecond and picosecond laser.Calculations show that under irradiation of nano-or picosecond laser pulses gold particles of submicrometer size can easily be heated to several hundred kelvins,the thermal effects is confined in a space less than tens of nanometers.The mechanism of denaturalized protein is analyzed with this laser-based absorpting nanopartide technology.