细菌视紫红质膜的光电荷转移 动力学过程的研究

实验测量了细菌视紫红质（bR）分别经波长为1064nm、532nm和355nm，脉冲宽度为10ns的三种不同的脉冲激光照射后产生的不同的光电压信号，着重用532nm脉冲光激发样品，从变化的测量回路的阻抗所得到的不同光电信号情况，研究并分析了细菌视紫红质的光电荷的产生和转移的微观机制。结果表明，其正负脉冲光电压信号是正负两种电荷移动的结果，而正电荷的产生比负电荷的产生和转移的微观机制。结果表明，其正负脉冲光电压信号是正负两种电荷移动的结果，而正电荷的产生比负电荷的产生需要克服更高的能量，同时，正负电荷既具有各自的转移特性又相互影响，如正电荷移动速度慢，负电荷移动速度快，负电荷对正电荷有牵引作用等，这些特性与电极和样品之间形成的界面效应有关，它使得细菌视紫红质的光电压信号的幅值和宽度对外接电阻的关系具有与其他光电响应器件所不同的特性。

The Kinetics of Photo-Charge Transfering in Bacteriorhodopsin

The different photoelectric signals in bacteriorhordopsin are experimentally measured when it is excited by 10 ns pulsee lasers at 1064 nm, 532 nm and 355 nm. 532 nm pulse laser is used mostly as pumping light to measure the photoelectric signals affected by the impedance of the measuring circuit. The mechanism that bacteriorhodopsin generates photo charges and the photo charges transfer in it was studied and analyzed. The results show that the positive and negative photoelectric signals are produced by the shift of the positive and negative charges respectively, and more energy is needed to produce positive charges than negative ones. At the same time, the positive and negative charges not only have their own transfering characteristics, but also affect each other, such as, positive charges move slowly, and negative ones fast, moreover, the negative can pull the positive to move forward, which, together with the interface between the sample and electrodes, cause the magnitude and width of the pulse photo voltage having special dependence different from other photo electronic devices on the linked resistance.