| 荧光共焦扫描系统成像特性的优化 |
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| 引用本文： | 张平,吴震.荧光共焦扫描系统成像特性的优化[J].光学学报,1997,17(3):08-313. |
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| 作者姓名： | 张平 吴震 |
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| 作者单位： | 华中理工大学光电子工程系 |
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| 摘 要： | 对荧光共焦扫描系统用光强点扩散函数进行傅里变换得到系统三维传递函数的数学模型，并由此求得环形透镜和各种有限大小探测器系统的光学传递函数。用计算机模拟和光学传递九数值计算，分析了采用不同环形透镜及探测器对系统成像特性的影响
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| 关 键 词： | 荧光 三维成像 共焦扫描 传递函数 信息光学 |
| 收稿时间： | 1996/2/9 |
Imaging Optimization of Fluorescence Confocal Scanning System |
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| Zhang Ping,Wu Zhen,Wang Cuiying,Xian Jiying.Imaging Optimization of Fluorescence Confocal Scanning System[J].Acta Optica Sinica,1997,17(3):08-313. |
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| Authors: | Zhang Ping Wu Zhen Wang Cuiying Xian Jiying |
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| Abstract: | The mathematical model of 3 dimensional optical transfer function (OTF) is obtained by Fourier transform of point spread function for a fluorescence confocal scanning microscopic system. From this, the 3 dimensional OTF is computed for different sized annular lenses and detectors. By using computer simulation and numerical computation of OTF, the image property is analyzed for the case of different annular lenses and detector in confocal scanning system. |
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| Keywords: | fluorescence 3 dimensional image confocal scanning transfer function |
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