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Labeling of macrophages using bacterial magnetosomes and their characterization by magnetic resonance imaging |
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| Authors: | Annegret Hartung Marcus R Lisy Karl-Heinz Herrmann Ingrid Hilger Dirk Schüler Claus Lang Matthias E Bellemann Werner A Kaiser Jürgen R Reichenbach |
| Institution: | 1. Medical Physics Group, Institute for Diagnostic and Interventional Radiology, Friedrich-Schiller University, Jena, Germany;2. Experimental Radiology, Institute for Diagnostic and Interventional Radiology, Friedrich-Schiller University, Jena, Germany;3. Institute for Diagnostic and Interventional Radiology, Friedrich-Schiller University, Jena, Germany;4. Department of Biomedical Engineering, University of Applied Sciences, Jena, Germany;5. Max-Planck Institute for Marine Microbiology, Bremen, Germany |
| Abstract: | This work investigated macrophages labeled with magnetosomes for the possible detection of inflammations by MR molecular imaging. Pure magnetosomes and macrophages containing magnetosomes were analyzed using a clinical 1.5 T MR-scanner. Relaxivities of magnetosomes and relaxation rates of cells containing magnetosomes were determined. Peritonitis was induced in two mice. T1, T2 and T2* weighted images were acquired following injection of the probes. Pure magnetosomes and labeled cells showed slight effects on T1, but strong effects on T2 and T2* images. Labeled macrophages were located with magnetic resonance imaging (MRI) in the colon area, thus demonstrating the feasibility of the proposed approach. |
| Keywords: | Magnetosome Macrophage Magnetic resonance imaging Molecular imaging Iron particle Inflammation |
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