Characterization of PEI-coated superparamagnetic iron oxide nanoparticles for transfection: Size distribution,colloidal properties and DNA interaction |
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| Authors: | Benedikt Steitz Heinrich Hofmann Sarah W Kamau Paul O Hassa Michael O Hottiger Brigitte von Rechenberg Magarethe Hofmann-Amtenbrink Alke Petri-Fink |
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| Institution: | 1. Laboratory of Powder Technology, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland;2. Institute of Veterinary Biochemistry and Molecular Biology, University of Zürich, Zürich, Switzerland;3. Musculoskeletal Research Unit, Equine Hospital, Vetsuisse Faculty Zurich, University of Zurich, Winterthurerstr. 260, 8057 Zurich, Switzerland;4. MatSearch, Chemin Jean Pavillard 14, 1009 Pully, Switzerland |
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| Abstract: | Superparamagnetic iron oxide nanoparticles (SPIONs) were coated with polyethylenimine. Here, we briefly describe the synthesis as well as DNA:PEI:SPION complexes and the characterization of the compounds according to their particle size, ζ-potential, morphology, DNA complexing ability, magnetic sedimentation, and colloidal stability. PEI coating of SPIONs led to colloidally stable beads even in high salt concentrations over a wide pH range. DNA plasmids and PCR products encoding for green fluorescent protein were associated with the described beads. The complexes were added to cells and exposed to permanent and pulsating magnetic fields. Presence of these magnetic fields significantly increased the transfection efficiency. |
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| Keywords: | Superparamagnetism Iron oxide Transfection Gene delivery Colloidal stability |
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