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Laccase immobilized on magnetic carriers for biotechnology applications |
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| Authors: | Jana Rotková Lucie Korecká Miroslava Jandová Daniel Horák |
| Institution: | a Department of Biological and Biochemical Sciences, University of Pardubice, Štrossova 239, 530 03 Pardubice, Czech Republic b Department of Technology of Organic Compounds, Doubravice 41, 533 53 Pardubice, Czech Republic c Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovského nám. 2, 162 06 Praha, Czech Republic |
| Abstract: | Laccase catalyzing the oxidation of p-diphenols has been applied in many industrial and biotechnology areas. Immobilized form of laccase has overcome the problem with contamination of the final product. Nevertheless sensitive enzymes immobilized to the matrix can be inactivated by the environmental conditions. The aim of this research was to prepare carrier with improved activity and responsible stability even under extreme reaction conditions. Laccase immobilized through carbohydrate moieties on magnetic hydrazide bead cellulose with a final activity of 0.63 I.U./1 ml of settled carrier confirmed that carriers with oriented immobilized enzyme might be useful in routine biocatalytic applications. |
| Keywords: | Macroporous bead cellulose Laccase Oriented immobilization Enzyme carrier Textile dye |
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