超声激活血卟啉对B16小鼠黑色素瘤细胞杀伤作用的研究初报

采用MTT法检测,经超声、超声 血卟啉分别作用后细胞线粒体内琥珀酸脱氢酶的活性,研究超声激活血卟啉对体外培养的B16小鼠黑色素瘤细胞的杀伤作用。结果表明:1.6MHz超声对B16小鼠黑色素瘤细胞辐照5min可表现出明显的杀伤效应,而超声协同被激活的血卟啉对B16细胞的杀伤作用比单纯超声的杀伤效应更加明显,且有显著性差异。

The lesion effect of ultrasound incorporation with Hematoporphyrin on mouse melanoma cells

In order to investigate the survival of mouse B16 melanoma cells suspended in different solutions and exposed to ultrasound of different frequencies, MTT test which had been proven to be effective to measure the survival of cells was applied to study the activity of succinate dehydrogenase of the mitochondria in the cells and to evaluate the lesion effect of ultrasound in the cells. The result showed that there were distinct killing effects when B16 cells were exposed to ultrasound of different frequencies for five minutes. These data indicate that B16 cells are more sensitive to frequency of 1.6MHz, compared with often tested various frequencies (2.0, 2.6, 3.0MHz) and are more distinct by injured when tested by ultrasound with hematoporphyrin than ultrasound alone.