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| 超声波对木瓜蛋白酶催化活性影响的机理研究 | |
| 引用本文： | 陈小丽,黄卓烈,何平,巫光宏,詹福建,丘泰球.超声波对木瓜蛋白酶催化活性影响的机理研究[J].应用声学,2008,27(6):469-474. |
| 作者姓名： | 陈小丽 黄卓烈 何平 巫光宏 詹福建 丘泰球 |
| 作者单位： | 1. 广东海洋大学水产学院,湛江,524088 2. 华南农业大学生命科学学院,广州,510642 3. 华南理工大学食品与生物工程学院,广州,510641 |
| 基金项目： | 国家自然科学基金，广东省高教厅科研项目 |
| 摘 要： | 木瓜蛋白酶经适当参数的超声波处理后酶活力提高。超声处理后酶的米氏常数Km变小,最大反应速率Vm也减小。超声处理后酶的紫外吸收光谱不变,荧光发射光谱也不改变,而差示光谱出现明显的正峰和负峰。研究结果表明,超声波处理后,木瓜蛋白酶的构型没有改变,而构象发生了变化。本文讨论了超声波影响木瓜蛋白酶活性的可能机理。 |
| 关 键 词： | 超声波 木瓜蛋白酶 酶活力 构象 |
Study on effect of ultrasound on catalysis activity of papain |
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| CHEN Xiao-Li,HUANG Zhuo-Lie,HE Ping,WU Guang-Hong,ZHAN Fu-Jian and QIU Tai-Qiu.Study on effect of ultrasound on catalysis activity of papain[J].Applied Acoustics,2008,27(6):469-474. | |
| Authors: | CHEN Xiao-Li HUANG Zhuo-Lie HE Ping WU Guang-Hong ZHAN Fu-Jian and QIU Tai-Qiu |
| Institution: | 1 College of Fisheries,Guangdong Ocean University,Zhanjiang 524088) (2 College of Life Science,South China Agricultural University,Guangzhou 510642) (3 College of Food and Bioengineering,South China University of Technology,Guangzhou 510641) |
| Abstract: | The papain activity increased after the papain was treated with ultrasound of appropriate parameters.Km and Vm of papain decreased after the treatment.Ultrasound did not change the ultraviolet absorption spectrum and fluorescence emission spectrum of papa- in,but positive peaks and negative peaks appeared in the differential ultraviolet spectrum of papain.The results indicated that conformation of papain was posiblely transformed by ultrasonic treatment.The mechanism of effect of ultrasound on papain activity was dis- cussed in this paper. |
| Keywords: | Ultrasound Papain Enzyme activity Conformation |
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