| 流动注射荧光分光光度法测定DNA的研究 |
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| 引用本文： | 方光荣,宋功武,李玲,詹红菊,李襄宏.流动注射荧光分光光度法测定DNA的研究[J].光谱学与光谱分析,2002,22(4):631-633. |
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| 作者姓名： | 方光荣 宋功武 李玲 詹红菊 李襄宏 |
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| 作者单位： | 湖北大学分析测试中心,湖北,武汉,430062 |
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| 基金项目： | 中国分析测试基金资助课题 |
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| 摘 要： | 本文研究了中药有效成分盐酸小蘖碱与DNA的荧光光谱性质 ,并应用流动注射技术测定DNA的浓度 ,该法线性范围 0～ 12 μg·mL-1 ,检出限为 ( 3σ) 7 3ng·mL-1 ,相对标准偏差≤ 3 1% ,应用于合成样品的测定 ,取得满意的结果
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| 关 键 词： | 流动注射 荧光分光光度法 测定 DNA 小蘖碱 荧光光谱 中药 相互作用 药理 分析 |
| 文章编号： | 1000-0593(2002)04-0631-03 |
| 修稿时间： | 2001年3月22日 |
Determination of DNA by Fluorimetry with Flow Injection |
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| Guang-rong Fang,Gong-wu Song,Ling Li,Hong-ju Zhan,Xiang-hong Li.Determination of DNA by Fluorimetry with Flow Injection[J].Spectroscopy and Spectral Analysis,2002,22(4):631-633. |
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| Authors: | Guang-rong Fang Gong-wu Song Ling Li Hong-ju Zhan Xiang-hong Li |
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| Institution: | Analysis and Testing Center of Hubei University, Wuhan, 430062 China. |
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| Abstract: | A highly sensitive fluorimetry determination of DNA is developed in this paper. The maximum fluorescence intensity was produced in the pH 3.05, with the maximum excitation and emission wavelength of 362 and 531 nm, respectively. Under the optimum conditions, the fluorescence intensity was proportional to the concentration of DNA. The linear range for calf thymus DNA is of 0-120 mg.L-1. The limit of determination was 0.07 mg.L-1. When the proposed method was used to determine DNA in the presence of some coexisting substances, a satisfactory result was obtained. |
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| Keywords: | Flow injection analysis DNA Fluorimetry |
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