基于多光谱成像技术的宫颈脱落细胞DNA定量分析研究

常用的宫颈癌筛查方法有TBS(the bethesda system)分类法和细胞DNA定量分析法两种,而同时利用多重染色方法在同一张细胞涂片上对细胞质进行巴氏染色和对细胞核进行Feulgen染色进行宫颈癌筛查的研究仍然是空白。多重染色筛查方法的难点在于非DNA物质的吸光度会干扰DNA物质的吸光度。因此建立了一套多光谱成像系统,并基于吸光度的线性叠加特性,利用多元线性回归方法建立了吸光度剥离模型,通过该模型成功地将DNA物质的吸光度剥离出来进行DNA定量分析,实现了两种常用方法的完美结合。通过一系列实验证明了,利用模型剥离出的DNA物质的吸光度与实测的DNA物质的吸光度在检验水平为1%的情况下在统计学上没有显著差异,而且实际应用测试中的统计数据也显示在置信水平为99%的情况下使用该分析方法筛查得到的四倍体细胞的DNA指数的置信区间与癌细胞的DNA指数判别区间没有交集,验证了这种基于多光谱成像技术的多重染色的DNA定量分析方法的准确性和可行性,在宫颈癌乃至其他癌症的早期诊断和筛查中有巨大的市场应用潜力与广阔的应用前景。

Study of Cervical Exfoliated Cell 's DNA Quantitative Analysis Based on Multi-Spectral Imaging Technology

The conventional cervical cancer screening methods mainly include TBS (the bethesda system ) classification method and cellular DNA quantitative analysis ,however ,by using multiple staining method in one cell slide ,which is staining the cyto-plasm with Papanicolaou reagent and the nucleus with Feulgen reagent ,the study of achieving both two methods in the cervical cancer screening at the same time is still blank .Because the difficulty of this multiple staining method is that the absorbance of the non-DNA material may interfere with the absorbance of DNA ,so that this paper has set up a multi-spectral imaging system , and established an absorbance unmixing model by using multiple linear regression method based on absorbance 's linear superpo-sition character ,and successfully stripped out the absorbance of DNA to run the DNA quantitative analysis ,and achieved the perfect combination of those two kinds of conventional screening method .Through a series of experiment we have proved that between the absorbance of DNA which is calculated by the absorbance unmixxing model and the absorbance of DNA which is measured there is no significant difference in statistics when the test level is 1% ,also the result of actual application has shown that there is no intersection between the confidence interval of the DNA index of the tetraploid cells which are screened by using this paper's analysis method when the confidence level is 99% and the DNA index's judging interval of cancer cells ,so that the accuracy and feasibility of the quantitative DNA analysis with multiple staining method expounded by this paper have been veri-fied ,therefore this analytical method has a broad application prospect and considerable market potential in early diagnosis of cer-vical cancer and other cancers .