| 甲胺磷人工抗原的合成及光谱表征 |
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| 引用本文： | 王向红,段振娟,宋诗莹,王硕.甲胺磷人工抗原的合成及光谱表征[J].光谱学与光谱分析,2007,27(5):881-885. |
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| 作者姓名： | 王向红 段振娟 宋诗莹 王硕 |
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| 作者单位： | 1. 天津市食品营养与安全重点实验室,天津科技大学生物技术与食品工程学院,天津,300457;河北农业大学食品科技学院,河北,保定,071001
2. 天津市食品营养与安全重点实验室,天津科技大学生物技术与食品工程学院,天津,300457 |
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| 基金项目： | 国家高技术研究发展计划(863计划)
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人事部高层次留学回国人才资助计划项目
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天津科技大学校科研和教改项目 |
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| 摘 要： | 通过化学方法合成了一种甲胺磷的半抗原6-(O,O-二甲基硫代磷酰基)氨基己酸(MC),MC与N-羟基琥珀酰亚胺(NHS)反应得到6-(O,O-二甲基硫代磷酰基)氨基己酸活化酯(MCE),活化酯(MCE)与匙孔血兰蛋白(KLH)偶联得到第一种人工抗原(MC-KLH);直接用甲胺磷作为半抗原用重氮化法与牛血清蛋白(BSA)偶联得到第二种人工抗原(M-BSA).通过质谱、红外和核磁表征,证明所合成的半抗原是设计的目标产物;并且对两种人工抗原进行了红外光谱分析,结果表明,人工抗原1的红外光谱中既有酰胺结构单元又有半抗原中的甲氧结构单元;半抗原2中不但具有酰胺结构单元又有甲胺磷的甲氧基和硫氧基结构单元,以此判断半抗原和蛋白偶合成功,为红外光谱法用于人工抗原合成与否的鉴定研究提供了依据.
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| 关 键 词： | 甲胺磷 半抗原 人工抗原 红外光谱法 |
| 文章编号： | 1000-0593（2007）05-0881-05 |
| 收稿时间： | 2005-12-10 |
| 修稿时间： | 2006-04-20 |
Spectral Analysis and Synthesis of Artificial Antigen of the Organophosphors Pesticide Methamidophos |
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| WANG Xiang-hong,DUAN Zhen-juan,SONG Shi-ying,WANG Shuo.Spectral Analysis and Synthesis of Artificial Antigen of the Organophosphors Pesticide Methamidophos[J].Spectroscopy and Spectral Analysis,2007,27(5):881-885. |
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| Authors: | WANG Xiang-hong DUAN Zhen-juan SONG Shi-ying WANG Shuo |
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| Institution: | 1. Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Food Science and Biologic Technology College, Tianjin 300457, China; 2. Agricultural University of Hebei, Food Science and Technology College, Baoding 071001, China |
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| Abstract: | In the present paper, a hapten of methamidophos was synthesized and conjugated with KLH by active ester method, thus the first artificial antigen was obtained. By diazotization method metharnidophos conjugated with BSA, and the second artificial antigen was obtained. The synthesized haptens were characterized by MS, IR and ^1 H NMR, and the two artificial antigens were determined by the method of IR spectrum. The result implied that both the artificial antigens have absorbance peaks of hapten and protein, indicating that they were prepared successfully. This could provide evidence that the method of IR spectrum can be used to determine whether the artificial antigens are synthesized successfully. |
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| Keywords: | Methamidophos Hapten Artificial antigen LR spectrum |
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