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乳腺组织形态基元共焦显微拉曼光谱的研究
引用本文:于舸,徐晓轩,吕淑华,张存洲,宋增福,张春平.乳腺组织形态基元共焦显微拉曼光谱的研究[J].光谱学与光谱分析,2006,26(5):869-873.
作者姓名:于舸  徐晓轩  吕淑华  张存洲  宋增福  张春平
作者单位:1. 北京石油化工学院数理教学部,北京,102617
2. 南开大学物理科学学院光子学中心,天津,300071
3. 天津医科大学肿瘤医院,天津,300060
4. 北京大学物理学院,北京,100871
摘    要:利用共焦显微拉曼方法,测定了乳腺组织切片的Mapping拉曼谱,光谱采样体积达到μm尺度.通过对这些共焦拉曼谱与商业提纯肌动蛋白、胶原等生化物质做出的谱的分析比较,分离出了来自细胞质、细胞间质、脂肪以及乳腺沉积物这些组织形态基元的拉曼谱;用K阶簇分析方法,还获得了细胞核基元谱;基元谱与提纯化学物质谱的相关分析印证了组织形态基元谱的来源.还分析总结了这些组织形态基元谱的特征.这些工作为深入理解组织形态与其拉曼谱的关系以及乳腺组织形态拉曼模型的建立奠定了基础.

关 键 词:共焦显微拉曼光谱  乳腺  组织形态基元  Mapping  K阶簇分析  相关分析
文章编号:1000-0593(2006)05-0869-05
收稿时间:2005-02-21
修稿时间:2005-05-22

Confocal Raman Microspectroscopic Study of Human Breast Morphological Elements
YU Ge,XU Xiao-xuan,L Shu-hua,ZHANG Cun-zhou,SONG Zeng-fu,ZHANG Chun-ping.Confocal Raman Microspectroscopic Study of Human Breast Morphological Elements[J].Spectroscopy and Spectral Analysis,2006,26(5):869-873.
Authors:YU Ge  XU Xiao-xuan  L Shu-hua  ZHANG Cun-zhou  SONG Zeng-fu  ZHANG Chun-ping
Institution:1. Department of Mathematics and Physics, Beijing Institute of Petrochemical Technology, Beijing 102617, China; 2. Photonics Center, Institute of Physics, Nankai University, Tianjin 300071, China ;3. Tumour Hosptial, Tianjin Medical University, Tianjin 300060, China; 4. College of Physics, Peking University, Beijing 100871, China
Abstract:Breast tissue sections were examined by means of confocal Raman spectroscopy with an excitation wavelength of 633 nm. Acquired using a microscopic mapping approach with the sample volume of -2 microm3, these spectra were compared with the ones of the commercially available actin, DNA, collagen (type I), triolein etc. Some spectra were distinguished and identified and characterize the morphological elements like cell cytoplasm, extracellular matrix etc. The cell nucleus spectrum was also obtained by K-means cluster analysis. The correlation analysis showed that the spectrum from a morphological element is highly correlated with that from the corresponding purified chemical. The spectroscopic characterization of these morphological elements was then investigated. This study is helpful to understanding the chemical/morphological basis of the Raman spectrum and designing the Raman microspectroscopic model of human breast tissue.
Keywords:Confocal Raman microspectroscopy  Breast  Morphological elements  Mapping  K-means cluster analysis  Correlation analyisis
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