真伪半夏二维相关红外光谱法的鉴别研究

本文采用红外光谱法(FTIR)并结合二维相关光谱分析技术(Two-Dimensional Correlation Spectroscopy)，对正品半夏和伪品水半夏进行了无损快速鉴别研究。半夏和水半夏在一维谱图上差别不显著，而在二维谱图上具有显著性的差别。在1 220～1 700 cm-1波段范围内，半夏在同步图上有1个较强的自动峰，在1 410 cm-1附近。而水半夏不仅在1 410 cm-1有一个比正品半夏较强的自动峰，还在1 540和1 680 cm-1附近出现了自动峰;同样在940～1 220 cm-1波段范围内半夏有3个较强的自动峰，分别在980，1 080和1 150 cm-1附近，而水半夏只有2个较强的自动峰，在1 080和1 150 cm-1附近。在980 cm-1处未出现自动峰。两个波段的异步谱也显示出较大的差别。结果表明：正品半夏和伪品水半夏的二维相关谱可以用于药材真伪品的鉴别依据。该法快速、准确，为中药材的快速鉴别提供了一种新的方法和手段。

Study on the Identification of Standard and False BanXia by Two-Dimensional Infrared Correlation Spectroscopy

Standard BanXia (Pinellia ternata (Thumb.) Breit) and false Shui BanXia (Typhonium flagelliforme (Lodd.) Blume) were identified fast and nondestructively by Fourier Transform Infrared Spectroscopy (FTIR) combined with two-dimensional correlation spectroscopy (2D) in this paper. The two kinds of BanXia wree similar to each other in one-dimensional IR spectra but quite different in 2D FTIR spectra. In the range between 1 220 cm -1 and 1 700 cm -1, one strong autopeak was aroused by BanXia around 1 410 cm -1 in synchronous spectrum, whereas three autopeaks not only at 1 460 cm -1 but also at 1 540 cm -1 and 1 680 cm -1 aroused by Shui BanXia. Also in the range between 940 cm -1 and 1 220 cm -1, three autopeaks were shown at 980, 1 080 and 1 150 cm -1 in synchronous spectrum of BanXia, but only two autopeaks at 1080 and 1 150 cm -1 in synchronous spectrum of Shui BanXia. It is proved that the 2D FTIR could serve in distinguishing standard and false medicinal herbs due to its unique advantages such as enhanced resolution and increased identification ability. Therefore, the rapidity and accuracy of 2D FTIR spectroscopy make it a powerful and new approach to evaluating the medicinal herbs.