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磷钼蓝分光光度法测定维生素C
引用本文:奚长生.磷钼蓝分光光度法测定维生素C[J].光谱学与光谱分析,2001,21(5):723-725.
作者姓名:奚长生
作者单位:韶关大学化学系,
基金项目:广东省高教厅自然科学基金资助 (粤高教科 [1 999] 89号 )
摘    要:基于在一定的反应条件下,维生素C可以定量地将磷钼酸锭还原成磷钼蓝,提出了一种新的测定维生素C的分光光度法。该方法很方便、快速地测定生物、药物等试样中的维生素C,准确度和重复性均达到令人满意的程度。

关 键 词:磷钼蓝  分光光度法  维生素C  磷钼酸铵  氧化
文章编号:1000-0593(2001)05-0723-03
修稿时间:1999年11月12

Determination of Vc with Phosphomolybdate-blue Spectrophotometry
C S Xi.Determination of Vc with Phosphomolybdate-blue Spectrophotometry[J].Spectroscopy and Spectral Analysis,2001,21(5):723-725.
Authors:C S Xi
Institution:Department of Chemistry, Shaoguan University, Shaoguan 512005, China.
Abstract:According as that Vc can reduce phosphomolybdate ammonium quantitatively to blue substance on certainl conditions,a new spctrophotometry determinating Vc is proposed in this paper.On the conditions selected in this paper,the maximum absorptive wavelength of phosphomolybdate-blue formed by the reaction of ammonium phosphomolybdate with Vc is at 700 1 nm.It does not form moly-blue(650 nm) when excessive ammonium phosphomolybdate react with trace Vc.The conditions of determination is easy to control.This method can determinate Vc in food,medicine easily and conveniently.The accuracy and the repeatability are satisfactory.
Keywords:Phosphomolybdate  Spectrophotometry  Vitamin C
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