原子荧光法测定刺五加不同部位中的砷、锑、汞、硒

采用微波消解处理样品,建立了氢化物发生-原子荧光法测定刺五加不同部位中的As,Sb,Hg,Se的分析方法。在最佳工作条件下,Se,Sb,Hg,Se的浓度与荧光强度均呈现良好的线性关系。砷的检出限为0.068 ng.mL-1,RSD为1.05%;锑的检出限为0.155 ng.mL-1,RSD为1.32%;汞的检出限为0.014 ng.mL-1,RSD为2.03%;硒的检出限为0.052 ng.mL-1,RSD为2.34%。并选用标准物质人发(GBW07601)对测定方法的准确度进行考察,该方法灵敏、快速准确。实验结果表明,刺五加不同部位中As,Sb,Hg,Se的含量有所差异。As,Hg和Se主要在叶中含量较高,锑在根中的含量高于其他部位。

Determination of Arsenic, Antimony, Mercury and Selenium in Different Parts of Acanthopanax Senticosus Harms by Atomic Fluorescence Spectrometry

A method for the determination of arsenic, antimony, mercury and selenium in different parts (root, stem, leaf and fruit) of aeanthopanax senticosus harms using hydride generation atomic fluorescence spectrometry with microwave digestion was developed. Under the optimized experimental conditions, the concentration of arsenic, antimony, mercury and selenium showed a linear relationship with the fluorescence intensity. The detection limit of arsenic was 0. 068 ng·mL~(-1), and the RSD was 1.05%. The detection limit of antimony was 0. 155 ng·m.L~(-1), and the RSD was 1.32%. The detection limit of mercury was 0. 014 ng·mL~(-1), and the RSD was 2.03%. The detection limit of selenium was 0. 052 ng·mL~(-1), and the RSD was 2.34%.The method was checked in terms of accuracy and precision using the standard reference substance of hair(GBW07601), and proved to be sensitive and rapid with satisfactory results. The content of arsenic, mercury and selenium is high in the leaf, and that of antimony is higher in the root than in other parts.