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藏红T荧光熄灭法测定痕量亚硝酸根
引用本文:董存智,吴伟东.藏红T荧光熄灭法测定痕量亚硝酸根[J].光谱学与光谱分析,2003,23(1):170-172.
作者姓名:董存智  吴伟东
作者单位:淮北煤炭师范学院化学系,安徽,淮北,235000
摘    要:研究了盐酸介质中亚硝酸根与藏红T发生的重氮化反应 ,基于藏红T的荧光强度随亚硝酸根的加入量增加而明显降低的现象 ,建立了荧光熄灭法测定痕量亚硝酸根的新方法。方法的激发波长为 52 5 0nm ,发射波长为 556 0nm。在盐酸浓度为 0 0 96mol·L- 1 ,反应时间为 1 0min的条件下 ,藏红T的荧光强度与亚硝酸根的加入量在 4~ 2 0 0× 1 0 - 9g·mL- 1 NO- 2 间存在线性关系 ,方法的检出限为 4× 1 0 - 9g·mL- 1 NO- 2 。方法操作简便、反应迅速、灵敏度高 ,已用于水样中痕量亚硝酸根的测定 ,并对反应的机理进行了讨论。

关 键 词:藏红T  亚硝酸根  荧光熄灭法
文章编号:1000-0593(2003)01-0170-03
修稿时间:2001年8月20日

Spectrophotofluorimetric Determination of Trace Nitrite with Safranine T
DONG Cun-zhi and WU Wei-dong.Spectrophotofluorimetric Determination of Trace Nitrite with Safranine T[J].Spectroscopy and Spectral Analysis,2003,23(1):170-172.
Authors:DONG Cun-zhi and WU Wei-dong
Institution:Department of Chemistry, Huaibei Coal Nolmal College, Huaibei 235000, China.
Abstract:A new fluorescence quenching method has been developed to determine nitrite in water samples. The method is based on the diazo-reaction between nitrite ion and safranine T in hydrochloric acid medium. The fluorescence intensity was measured at excitation and emission wavelengths of 525.0 and 556.0 nm, respectively. The optimum experimental conditions have been confirmed and the concentration of hydrochloric acid was 0.096 mol.L-1; the reaction time was 10 min. Under the selected conditions, the relationship was obtained between the fluorescence intensity and nitrite concentration in the range 4-200 x 10(-9) g.mL-1 NO2-. The detection limit was 4 x 10(-9) g.mL-1 NO2-. The method was applied to determine trace nitrite in water samples. The mechanism involved in the reaction was discussed.
Keywords:Safranine T  Nitrite  Spectrophotofluorimetry
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