氢化物发生-原子荧光光谱法研究嗜热四膜虫对阿散酸饲料添加剂及其环境降解物的砷吸收

用以单细胞原生动物嗜热四膜虫对阿散酸及其降解物的砷吸收模型,研究了饲料添加剂阿散酸及其环境降解物对生物体的影响.四膜虫在含有阿散酸及其降解物的培养基中培养72 h后,洗去培养基,收集细胞.将虫体裂解,分离细胞膜和细胞内质液,经消解.用流动注射氢化物发生-原子荧光光谱法(HG-AFS)分别测定了细胞膜上和细胞膜内的砷.结果表明,阿散酸及其环境降解物可被细胞膜吸收并进入细胞内,且降解产生的无机砷比阿散酸更易被吸收,生物体通过细胞的砷吸收引起毒害.

Research on total arsenic absorption of tetrahymena pyriformis exposed to arsanilic acid feed additive and its environmental degradation products by HG-AFS

In the present paper, a model based on the arsenic absorption of the unicellular microorganism tetrahymena pyriformis from the incubation aqueous solution of feed additive arsanilic acid and its degradation products As(III) and As(V) was established for the investigation of the environmental impacts of feed additive arsanilic acid on the living organisms. The tetrahymena pyriformis was incubated in the incubation aqueous solution and its degradation products for 72 h, after cleaning to remove the incubation aqueous solution, the cell was broken up and separated. The cell membrane and cytoplasm were digested with nitric acid and hydrochloric acid separately. A method of arsine generation atomic fluorescence spectrometry was used for the determination of the absorbed arsenic. Experimental findings illustrate that arsenilic acid and its degradation products As(III) and As(V) can be absorbed by the cell membrane then enter into cytoplasm. In addition, the degradation products As(III) and As(V) would more easily be absorbed than the arsanilic acid. The living organisms would be poisoned via the As absorption of the cells.