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有/无金属离子Cu2+或Zn2+参与时中药有效成分伞形花内酯与生物大分子BSA的相互作用
引用本文:刘雪锋,夏咏梅,方云,邹鲁,刘玲玲.有/无金属离子Cu2+或Zn2+参与时中药有效成分伞形花内酯与生物大分子BSA的相互作用[J].光谱学与光谱分析,2005,25(8):1301-1305.
作者姓名:刘雪锋  夏咏梅  方云  邹鲁  刘玲玲
作者单位:江南大学化学与材料工程学院,江苏,无锡,214036
基金项目:江苏省科委基金(BJ99036),江南大学自然科学教研基金(2004LY019)资助项目
摘    要:运用荧光光谱(FS)及紫外光谱(UV)研究了有/无金属离子Cu2 或Zn2 参与时中药有效成分伞形花内酯与生物大分子牛血清白蛋白(BSA)的相互作用。实验结果表明:有/无金属离子参与时,伞形花内酯均与BSA形成基态复合物从而猝灭BSA的内源性荧光,猝灭原因主要为静态猝灭和非辐射能量转移;金属离子参与使得KA增大,n仍维持在2左右;伞形花内酯分子能够扦插入BSA分子内部,温度以及金属离子参与对伞形花内酯与BSA分子中荧光性氨基酸残基间的空间距离r影响不大。有/无金属离子参与时伞形花内酯与BSA的作用过程均是一个熵增加和Gibbs自由能降低的自发超分子作用过程。伞形花内酯与BSA之间以静电相互作用为主,金属离子参与使伞形花内酯与BSA分子间静电相互作用增强,故ΔH对ΔG的贡献增大。

关 键 词:中药有效成分  伞形花内酯  牛血清白蛋白  金属离子  荧光光谱法
文章编号:1000-0593(2005)08-1301-05
收稿时间:2003-12-08
修稿时间:2004-03-28

Interaction between Bovine Serum Albumin and Umbelliferone with/without Metal Ions of Cu2+ or Zn2+
LIU Xue-feng,XIA Yong-mei,FANG Yun,ZOU Lu,LIU Ling-ling.Interaction between Bovine Serum Albumin and Umbelliferone with/without Metal Ions of Cu2+ or Zn2+[J].Spectroscopy and Spectral Analysis,2005,25(8):1301-1305.
Authors:LIU Xue-feng  XIA Yong-mei  FANG Yun  ZOU Lu  LIU Ling-ling
Institution:School of Chemical and Material Engineering, Southern Yangtze University, Wuxi 214036, China.
Abstract:The interaction between bovine serum albumin (BSA) a nd umbelliferone, an active component of Chinese herbs, with or without metal ions was investigated using fluorescence spectroscopy (FS) and UV. The experimental results showed that the umbelliferone molecules inserted into the hydrophobic pockets of BSA and quenched the intrinsic fluorescence of BSA by forming umbelliferone-BSA complex. The mechanism of fluorescence quenching was confirmed combining by both static quenching and nonradiative energy transferring. It was discovered that the apparent association constant (K(A)) increases in the presence of Cu2+ and Zn2+, whereas the spatial-distance (r) between umbelliferone molecule and fluorescent amino acid residues of the BSA and the binding sites (n) of umbelliferone molecules on BSA have no obviously change. Binding of umbelliferone molecules to BSA was a spontaneous supramolecular interaction in which entropy increased and Gibbs free energy decreaed. The interaction of the umbelliferone-BSA was driven mainly by electrostatic force which was enhanced by Cu2+ and Zn2+, thus the contribution of AH to AG increased in the presence of metal ions.
Keywords:Active component of Chinese herbs  Umbelliferone  Bovine serum albumin  Metal ion  Fluorescence spectroscopy
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