| 荧光法研究Pb2+与牛血清白蛋白的相互作用 |
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| 引用本文： | 吴根华,汪春华.荧光法研究Pb2+与牛血清白蛋白的相互作用[J].光谱学与光谱分析,2005,25(2):246-248. |
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| 作者姓名： | 吴根华 汪春华 |
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| 作者单位： | 安庆师范学院化学与环境科学学院,安徽,安庆,246003;南开大学化学学院,天津,300071 |
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| 基金项目： | 国家自然科学基金(2017001),安徽省教育厅自然科学基金(2002kj201)资助课题 |
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| 摘 要： | 在模拟生理条件下, 用荧光光谱法研究了Pb2+与牛血清白蛋白(BSA)的相互作用. 结果表明 BSA分子中色氨酸和酪氨酸残基具有荧光发射性质, 以283 nm激发BSA, 在341 nm处有很强的荧光发射. 在加入Pb2+ 后, 发现Pb2+-BSA强荧光峰的位置不变, 但荧光强度随着Pb2+浓度的增大而明显减弱, 说明Pb2+对BSA有荧光猝灭现象, 猝灭以静态猝灭为主, 由Stern-Volmer方程求得Pb2+表观猝灭常数Kq=9.5×1012 L·mol-1·s-1. Pb2+主要与BSA中的N配位形成21配合物, 表观络合常数lgK=11.61.
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| 关 键 词： | BSA 荧光光谱法 Pb2+ |
| 文章编号： | 1000-0593(2005)02-0246-03 |
| 修稿时间： | 2004年3月16日 |
Study on the Interaction of Pb2+ and Bovine Serum Albumin by Fluorescence |
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| WU Gen-hua,WANG Chun-hua.Study on the Interaction of Pb2+ and Bovine Serum Albumin by Fluorescence[J].Spectroscopy and Spectral Analysis,2005,25(2):246-248. |
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| Authors: | WU Gen-hua WANG Chun-hua |
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| Institution: | Department of Chemistry, Anqing Teachers College, Anqing 246003, China. |
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| Abstract: | The interaction of Pb2+ and bovine serum albumin (BSA) was studied under conditions similar to those in human bodies by fluorescence spectra. The results indicated that tryptophan and tyrosine, which were located in BSA, had a max fluorescence emission peak at 341 nm with an excitation wavelength of 283 nm. It was shown that Pb2+ had a powerful ability to quench the BSA fluorescence with a mechanism of a static process rather than a dynamic one. The apparent quenching constant Kq was obtained to be 9.5 x 10(12) L x mol(-1) x s(-1) by Stern-Volmer equation. The apparent complexation constant of Pb2 x BSA is lgK = 11.61. The nitrogen in BSA could coordinate with lead in Pb2-BSA. |
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| Keywords: | BSA Fluorescence spectrum Pb~(2+) |
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