| 过硒二苯2,2'-二甲酸与牛血清白蛋白结合反应热力学特征研究 |
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| 引用本文： | 颜承农,上官云凤,刘义,侯安新,屈松生.过硒二苯2,2''''-二甲酸与牛血清白蛋白结合反应热力学特征研究[J].光谱学与光谱分析,2005,25(9):1486-1489. |
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| 作者姓名： | 颜承农 上官云凤 刘义 侯安新 屈松生 |
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| 作者单位： | 1. 长江大学化学与环境工程学院,湖北,荆州,434020
2. 武汉大学化学与分子科学学院,湖北,武汉,430072 |
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| 基金项目： | 国家自然科学基金（30170010）和湖北省自然科学基金（2005ABA067）资助 |
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| 摘 要： | 用荧光光谱和紫外．可见吸收光谱法研究了在不同温度下，新合成的过硒二苯2，2’-二甲酸（PSD）对牛血清白蛋白（BSA）作用的荧光猝灭光谱行为。分别用Stem-Volmer方程和Lineweaver-Burk双倒数方程处理试验数据，证实在试验浓度和温度范围内，其荧光猝灭作用更符合静态猝灭作用特征，PSD与BSA可反应并结合形成具有一定结构的复合物，得到了反应的结合常量、结合热力学性质和结合位点等参数；同时探讨了静态猝灭作用的机理和结合力的性质，为了解PSD与BSA的结合方式、在人体内的传输机理和药理作用提供科学依据。
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| 关 键 词： | 过硒二苯2，2’-二甲酸 BSA 荧光猝灭光谱 热力学参数 |
| 文章编号： | 1000-0593（2005）09-1486-04 |
| 收稿时间： | 2004-02-28 |
| 修稿时间： | 2004-06-06 |
Thermochemical Studies on the Binding Reaction of Perseleno Diphenyl 2,2'-Diformic Acid with Bovine Serum Albumin |
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| YAN Cheng-nong,SHANGGUAN Yun-feng,LIU Yi,HOU An-xin,QU Song-sheng.Thermochemical Studies on the Binding Reaction of Perseleno Diphenyl 2,2''''-Diformic Acid with Bovine Serum Albumin[J].Spectroscopy and Spectral Analysis,2005,25(9):1486-1489. |
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| Authors: | YAN Cheng-nong SHANGGUAN Yun-feng LIU Yi HOU An-xin QU Song-sheng |
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| Institution: | 1. College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou 434020, China; 2. College of Chemistry and Molecule Sciences, Wuhan University, Wuhan 430072, China |
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| Abstract: | The binding of newly compounded perseleno diphenyl 2,2'-diformic acid to bovine serum albumin(BSA) was studied at different temperatures using fluorescence spectrum and UV spectrum. The fluorescence quenching data was analyzed according to Stern-Volmer equation and Lineweaver-Burk double-reciprocal equation. It was showed that this quenching complies better with the charactristic of static fluorescence quenching. The binding constant, thermodynamic parameters, and the binding spot of the compound with certain structure, coming from perseleno diphenyl 2,2'-diformic acid and bovine serum albumin, were obtained. Besides, the mechanism of static fluorescence quenching and the quality of binding power were both discussed. The information of the binding mode, the mechanism of its transportation, and some medical theories in human body were offered. |
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| Keywords: | Perseleno diphenyl 2 2'-diformic acid Bovine serum albumin Fluorescence spectrum Thermodynamic parameters |
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