FTIR光谱方法对比分析少年和老年软骨成分含量

利用傅里叶变换红外(FTIR)光谱方法测量了少年和老年人软骨的红外光谱.由OMNIC 5.2软件计算了软骨中主要有机成分(胶原)和无机成分相关光谱带的积分面积.对代表有机物和无机物的某些谱带面积大小进行了比较.结果表明:软骨中有机成分(酰氨Ⅰ)含量与无机成分(PO34-)含量之比少年是老年人的5～7倍;然而脂类含量却有很大的不同,对脂类(1 747 cm-1)与无机物(PO34-)的谱带积分面积进行比较,老年人软骨中脂类含量明显高于少年.作者认为人体随着年龄的增加,全身性的退化在软骨中的总体表现为有机成分(胶原)的减少(而脂类含量却增加)和无机成分(磷酸盐、碳酸盐等)相对的增加,使得软骨骨质变脆,失去过多弹性,耐磨性减低,是进一步容易导致骨病和骨伤的原因.

FTIR Spectroscopic Investigations on the Difference in Cartilage Composition between Youth and Elder

Cartilage of youth and elder was measured by Fourier transform infrared (FTIR). Area integrations were done on the major composition of organic and inorganic substance's infrared bands processed using OMNIC 5. 2. Calculation of the ratio of the integrated areas under the organic and inorganic substance's peaks allowed the comparisons of the ratio brtween organic and inorganic substance's contents. As indicated from the results, the ratio of organic to inorganic substance in youth is 5-7 times larger than that in elder, but there was a big difference in the content of lipids between youth and elder. Calculating the ratio of the integrated areas under lipids peaks (1 747 cm(-1)) to that under inorganic phosphates peaks, the authors can see that the content of lipids in the bone of elder is significantly higher than that of youth. The authors admitted that with the increment of age, less organic substance (collagen) (but more lipids) and more inorganic substance (phosphate, carbonate etc) is a symbol of the degeneration of cartilage. Therefore, makes the cartilage more brittle, less elastic and less wearable. This can result in the disease of bone and injuring the bone easily.