首页 | 本学科首页   官方微博 | 高级检索  
     检索      

超临界流体CO2萃取中药有效成分的抗氧化性的分光光度法研究
引用本文:张立伟,王金山,江崇球.超临界流体CO2萃取中药有效成分的抗氧化性的分光光度法研究[J].光谱学与光谱分析,2004,24(9):1103-1105.
作者姓名:张立伟  王金山  江崇球
作者单位:山东师范大学化学系,山东,济南,250014
摘    要:利用超临界流体二氧化碳萃取技术提取中药虎杖、大黄、炒白术中黄酮类化合物。在pH8.2的HCl—tris缓冲溶液中利用邻苯三酚自氧化法测定提取物对超氧阴离子自由基的消除作用。用茜素紫作显色剂的分光光度法在pH9.0的HCl—tris缓冲体系中测定中药提取物对Co^2 H2O2体系所产生的羟基自由基的消除作用,并对实验条件进行了探讨。实验结果表明,3种中药提取物对自由基都有消除作用,其中虎杖的效果最好。

关 键 词:中药  有效成分  抗氧化性  分光光度法  超临界流体二氧化碳萃取
文章编号:1000-0593(2004)09-1103-03
修稿时间:2002年11月20

Spectrophotomatric Determination of Antioxidative Activity of Extractives of Chinese Traditional Medicine by Supercritical Fluid CO2 Extraction Technology
Li-wei Zhang,Jin-shan Wang,Chong-qiu Jiang.Spectrophotomatric Determination of Antioxidative Activity of Extractives of Chinese Traditional Medicine by Supercritical Fluid CO2 Extraction Technology[J].Spectroscopy and Spectral Analysis,2004,24(9):1103-1105.
Authors:Li-wei Zhang  Jin-shan Wang  Chong-qiu Jiang
Institution:Shandong Normal University, Ji'nan 250014, China.
Abstract:Applying supercritical CO 2 fluid extraction technology, flavonoids were extracted from Chinese traditional medicine asarum heterotropoides, atractylodes macrocephala, and rheum palmatun. Using the method of autoxidation pyrogallol (known as 325 nm method), the superoxide radical scavenging effect of the extraction was carried out in the buffer solution of HCl-tris(pH 8.2). With spectrophotometry, hydroxyl radical created by the system Co 2++H 2O 2 in the reaction like Fenton reaction was eliminated by alizarin violet as the color developing agent in the buffer solution HCl-tris (pH 9.0) and the reaction condition was investigated. Result showed that these extractions are elimination agent for these radicals. Asarum heterotropoides is the best of the three.
Keywords:Supercritical fluid extraction technology  Asarum heterotropoides  Atractylodes macrocephala  Rheum palmatun  Superoxide radical  Hydroxyl radical
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号