活体浮游植物同步荧光光谱特征分析研究

测量了东海五种常见浮游植物在3个温度(25,20,15℃)、3个光照(7 000,4 100,1 100 Lx)下的不同生长期的同步扫描荧光光谱,对其进行了多项式平滑和归一化处理,考察了同种藻的光谱相似性,并运用主成分分析确立了其标准谱.分析结果表明,中肋骨条藻(Skeletonema costatuma,Sk)、等鞭金藻(Isoch-rysis galbana,Is)、岛国大扁藻(Platymonas helgolanidica,Pl)的特征光谱相似度高,塔玛亚历山大藻(Al-exandrium tamarense)和裸甲藻(Gymnodinium stein)的光谱相似度稍差.实验所用的硅藻中肋骨条藻与甲藻塔玛亚历山大藻、裸甲藻的标准光谱明显不同.

Research on Characterization Analysis of Synchronous Fluorescence Spectra of Living Phytoplankton

The synchronous fluorescence spectra of five phytoplankton species growing under three temperatures (25, 20 and 15 degrees C)and three illuminations (7000, 4100 and 1100 Lx)were measured and processed by multinomial smoothness and autoscaling to obtain the characteristic spectra. Principal component analysis was used to obtain standard spectra. The analysis shows that at different temperatures, the characteristic spectra of Skeletonema costatuma, Isochrysis galbana, and Platymonas helgolanidica show high similarities, while the spectra similarities of Alexandrium tamarense and Gymnodinium stein are not as good as the above three species. The standard spectrum of Skeletonema costatuma, which belongs to Bacillariophyta, is quite different from those of Alexandrium tamarense and Gymnodinium stein, which belong to Dinophyta.