色氨酸的非线性分频荧光研究

色氨酸 (Trp)在 350nm处产生一个荧光峰 ,在 70 0nm处产生一个 (分频 )荧光峰 ,此两峰荧光强度F3 50nm 和F70 0nm 均与Trp浓度 ( 0～ 1× 10 -5mol·L-1 )成线性关系 ,随着Trp浓度增大 ,350和 70 0nm半峰宽(Δλ) 3 50 ,(Δλ) 70 0 缓慢减小 ,而F70 0nm/F3 50nm 值和半峰宽比 (Δλ) 70 0 /(Δλ) 3 50 为一常数 ,此两峰具有相似的荧光特性。根据建立的分频荧光能级原理和非线性共振分频荧光原理探讨了色氨酸分频荧光峰产生的原因

A Study of Nonlinear Fraction Frequency Fluorescence Spectra for Tryptophane

There are two fluorescence peaks at 350 and 700 nm for tryptophane in pH 7.43 Tris buffer solution, as the excited wavelength is at 296 nm. The fluorescence peak at 700 nm is a nonlinear 1/2 fraction frequency fluorescence peak for tryptophane. The fluorescence intensities of 350 and 700 nm peaks are proportional to tryptophane concentration in the range of 0-1 x 10(-5) mol.L-1. The half width of (delta lambda)350 for 350 nm and (delta lambda)700 for 700 nm peak decreases slowly with tryptophane concentration. And the F700 nm/F350 nm and (delta lambda)700/(delta lambda)350 are constant. The two peaks have similar fluorescence behavior. An energy level principle for fraction frequency fluorescence and resonance principle of fraction frequency have been proposed, and applied to explaining the fraction frequency fluorescence of tryptopane.