高效液相色谱及联用技术在砷形态分析中的应用

砷与人类的生活息息相关,是环境中存在的一种极为重要的非金属元素.由于不同形态的砷其毒性各不同,因此无论对环境样品、食品、药物还是对人体的体液进行砷的形态分析都是至关重要的.高效液相色谱因具有较高的分离能力而被广泛地应用于砷形态的分离研究中.随着对分析灵敏度与准确度要求的不断提高,作为砷形态分析的检测器不断发展,不同的联用技术也日益完善.其中,电感耦合等离子体质谱法以其极高的灵敏度、多元素同时检测能力、极宽的动态范围以及同位素比检测能力而被成功地作为高效液相色谱的检测器,广泛地应用于砷的形态分析中.

Application of High Performance Liquid Chromatography and Hyphenated Techniques in Analysis of Arsenic Species

Arsenic is a very important nonmetal element in the environment and is vitally related to human lives. It is well known that the biological availability and toxicological effects of arsenic compounds depend upon their chemical forms, so it is necessary to detect the species of arsenic in some environmental samples, food, medicine, and body fluids. High performance liquid chromatography (HPLC) is widely used in the research on arsenic species because of its high separation capability. The detection techniques used for arsenic species analysis have been improved rapidly with the gradually increasing demand for sensitivity and accuracy. Various hyphenated techniques have also been developed. Inductively coupled plasma mass spectrometry has many advantages, such as high sensitivity, simultaneous multi-element detection, wide linear range and so on. Therefore, high performance liquid chromatography-inductively coupled plasma mass spectrometry is widely used in the analysis of arsenic species.