| 靶向分子羧甲基多糖与DNA作用机理研究 |
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| 引用本文： | 张海容,郭祀远,李琳,蔡妙颜.靶向分子羧甲基多糖与DNA作用机理研究[J].光谱学与光谱分析,2006,26(4):711-714. |
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| 作者姓名： | 张海容 郭祀远 李琳 蔡妙颜 |
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| 作者单位： | 1. 忻州师范学院生化分析技术研究所, 山西 忻州 034000
2. 华南理工大学轻工与食品学院，广东 广州 510640 |
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| 基金项目： | 山西省留学回国人员科研项目
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山西省高等学校科研开发基金 |
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| 摘 要： | 以溴化乙锭(EB)为荧光探针，结合紫外光谱法研究了壳聚糖、羧甲基壳聚糖、羧甲基纤维素与DNA的相互作用，药物分子与DNA作用程度可用作用常数D来表示，实验表明: 几种生物多糖与DNA分子的作用强弱顺序为: 壳聚糖＞羧甲基壳聚糖＞羧甲基纤维素;多糖主要以嵌插方式与DNA作用, 使EB-DNA荧光猝灭。
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| 关 键 词： | 荧光探针 羧甲基多糖 脱氧核糖核酸 作用常数 |
| 文章编号： | 1000-0593（2006）04-0711-04 |
| 收稿时间： | 2004-11-16 |
| 修稿时间： | 2005-04-06 |
Study on the Interaction Mechanism of Targeting Molecules Carboxymethyl Polysaccharide with DNA |
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| ZHANG Hai-rong,GUO Si-yuan,LI Lin,CAI Miao-yan.Study on the Interaction Mechanism of Targeting Molecules Carboxymethyl Polysaccharide with DNA[J].Spectroscopy and Spectral Analysis,2006,26(4):711-714. |
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| Authors: | ZHANG Hai-rong GUO Si-yuan LI Lin CAI Miao-yan |
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| Institution: | 1. Laboratory of Biochemical Analysis, Xinzhou Teacher’s University, Xinzhou 034000, China2. College of Light Industry and Food Science，South China University of Technology，Guangzhou 510640, China |
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| Abstract: | The interaction of chitosan(CTS),carboxymethyl chitosans(CMCTS),and carboxymethyl cellulose(CMCL) with DNA was investigated through ethidium bromide(EB) as a sensitive fluorescence probe,and ultraviolet absorption spectra.The constant D can directly denote the degree of interaction of polysaccharide with DNA.The results indicated that the interraction sequence of biological polysaccharides with DNA is as follows: CTS>CMCTS>CMCL.The polysaccharides were mainly bound to DNA by intercalated binding,which resulted in fluorescence quenching effect of EB-DNA. |
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| Keywords: | Fluorescence probe Carboxymethyl polysaccharide Deoxyribonucleic acid Function constant |
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