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FTIR光谱法研究天花粉蛋白的热去折叠过程
引用本文:卞为东,孙素琴,黄岳顺,周群,胡鑫尧.FTIR光谱法研究天花粉蛋白的热去折叠过程[J].光谱学与光谱分析,2000,20(4):471-473.
作者姓名:卞为东  孙素琴  黄岳顺  周群  胡鑫尧
作者单位:1. 清华大学化学系,100084,北京
2. 香港浸会大学生物系,香港
基金项目:清华大学、香港浸会大学、北京中医药大学中医药合作创意小课题资助
摘    要:本文利用FTIR光谱技术和计算机辅助解析技术(二阶导数、去卷积和曲线拟合)研究了天花粉蛋白的热诱导去折叠过程。结果表明:在25~85℃温度范围内,天花粉蛋白的热去折叠是一个不可逆的分子间聚集的过程;二级结构随温度的变化暗示了折叠中间体的存在。

关 键 词:去折叠  天花粉蛋白  热诱导  溶液构象  FTIR光谱

Study on Thermal Unfolding Process of Trichosanthin by FTIR Spectroscopy
Weidong BIAN,Suqin SUN,HUR.NS.WONG,Qun ZHOU,Xingyao HU.Study on Thermal Unfolding Process of Trichosanthin by FTIR Spectroscopy[J].Spectroscopy and Spectral Analysis,2000,20(4):471-473.
Authors:Weidong BIAN  Suqin SUN  HURNSWONG  Qun ZHOU  Xingyao HU
Institution:Department of Chemistry, Tsinghua University, 100084 Beijing.
Abstract:Fourier-transform infared spectroscopy, combined with resolution-enhancement techniques including second-derivative spectroscopy, Fourier self-deconvolution and curvefitting technique, was used to investigate the thermally induced unfolding process of anti-HIV-I toxin protein trichosanthin. During heating from 25 degrees C to 85 degrees C, the peak of Amide I shifted to 1618 cm-1 while the secondary structural contents change with the temperature. Upon cooling the protein from 85 degrees C to 25 degrees C, the contour of the Amide I do not change. All these show that the thermal unfolding of trichosanthin is an irreversible intermolecular aggregation process between 25 degrees C and 85 degrees C. The changes of secondary structures with temperature suggest the presence of folding intermediates.
Keywords:Second-derivative spectroscopy    Self-deconvolution    Unfolding    Curve-fitting  
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