木质素降解产物酚羟基测定方法研究进展

木质素降解是生物质资源化利用的一个重要方式，木质素本身是通过醚键或碳碳键连接的三维网状大分子结构，其官能团众多冗杂。当使用不同方法降解木质素时，检测降解木质素中酚羟基的含量可以直观地展现此方法对其降解的效率，并且能够反映木质素中的特定结构以及可水解、氧化与还原的活性等。提出并优化一种可以高效快速确定降解木质素中酚羟基浓度的测量方法，对解析解聚后木质素小分子的结构与功能很关键。根据对测试所用仪器的分类，综述了近年来利用滴定、紫外可见光度（差示紫外分光光度和Folin-Ciocalteu试剂等）、高效液相色谱、核磁共振（磷谱、碳谱、氢谱和氟谱等）、气相色谱-质谱以及气相色谱（氨解法测量1-乙酰吡咯烷、高碘酸盐氧化法测量甲醇）等木质素降解后酚羟基的检测方法与其实验或操作条件，并对每个定量分析方法的适用条件、样品要求和关键因素等进行解析，在基于高效、便捷、经济检测酚羟基的方法的前提下，展望了未来的发展方向。

Research Progress in Quantitative Determination of Phenolic Hydroxyl Groups in Lignin

Lignin degradation is an important way to the utilization of biomass resources. Lignin is a three-dimensional networked macromolecular structure with many functional groups. When different methods are used to degrade lignin, detecting the content of phenolic hydroxyl groups in degraded lignin can more intuitively show the degradation efficiency of this method and can reflect the specific structure of lignin and the activities of hydrolysis, oxidation and reduction. Designing or comparing a measurement method that can efficiently and quickly determine the concentration of phenolic hydroxyl groups in degraded lignin is critical for analyzing the structure and function of small lignin molecules after depolymerization. According to the classification of instruments, the recent detection methods of phenolic hydroxyl groups after the degradation of lignin, such as titration methods, ultraviolet spectroscopy (ultraviolet spectrophotometry and Folin-Ciocalteu reagent, etc.), high-performance liquid chromatography, nuclear magnetic resonance (phosphorus spectrum, carbon spectrum, hydrogen spectrum, fluorine spectrum, etc.), gas chromatography-mass spectrometry, and gas chromatography (measurement of 1-acetylpyrrolidine by ammonolysis and methanol by periodate oxidation) were reviewed. Moreover, the application conditions, sample requirements and key factors of each quantitative analysis method were analyzed. Based on the efficient, convenient and economical detection method of phenolic hydroxyl, the future development direction prospected.