| 催化荧光法测定痕量草酸 |
| |
| 引用本文： | 陈兰化,赵丹华.催化荧光法测定痕量草酸[J].光谱学与光谱分析,2003,23(1):203-204. |
| |
| 作者姓名： | 陈兰化 赵丹华 |
| |
| 作者单位： | 淮北煤炭师范学院化学系,安徽,淮北,235000 |
| |
| 摘 要： | 在硫酸介质中 ,草酸能催化重铬酸钾氧化罗丹明B褪色 ,使体系荧光猝灭 ,建立了催化荧光法测定草酸的新方法。催化反应在 50℃水浴中进行 1 0min ,为假零级反应。测定草酸的线性范围为 0 4～ 1 2 0mg·L- 1 ,其回归方程为ΔF =8 42c(mg·L- 1 ) 3 81 ,r=0 9983 ,检出限为 0 1 6mg·L- 1 。试验了 30种共存物质的干扰情况。此法用于生物样品中草酸含量的测定 ,结果满意。
|
| 关 键 词： | 草酸 罗丹明B 催化荧光法 |
| 文章编号： | 1000-0593(2003)01-0203-02 |
| 修稿时间： | 2001年6月18日 |
Catalytic Fluorimetric Determination of Trace Oxalate |
| |
| CHEN Lan-hua and ZHAO Dan-hua.Catalytic Fluorimetric Determination of Trace Oxalate[J].Spectroscopy and Spectral Analysis,2003,23(1):203-204. |
| |
| Authors: | CHEN Lan-hua and ZHAO Dan-hua |
| |
| Institution: | Department of Chemistry, Huaibei Coal Normal College, Huaibei 235000, China. |
| |
| Abstract: | A new catalytic fluorimetric method for the determination of trace oxalate has been developed. The method is based on the catalytic effect of oxalate on the oxidation of potassium dichromate with rhodamine B in sulfuric acid medium. The catalytic reaction takes place at 50 degrees C for 10 min in a water bath and is a pseudo-zero-order reaction. The regression equation of calibration graph is delta F = 8.42c (mg.L-1) + 3.81 (r = 0.9983), and measuring range is 0.4-12.0 mg.L-1, and the detection limit is 0.16 mg.L-1. Interferences of 30 diverse materials were examined. The method has been applied to the determination of oxalate in biological samples with satisfactory results. |
| |
| Keywords: | Oxalate Rhodamine B Catalytic fluorimetry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
