光谱法和分子对接法研究盐酸氨溴索与人血清白蛋白的相互作用

在模拟生理条件下,用光谱法和分子对接技术研究了盐酸氨溴索与人血清白蛋白的相互作用.不同温度下的Steen-Volmer曲线和紫外-可见吸收光谱表明:盐酸氨溴索主要以动态猝灭方式使人血清白蛋白的荧光猝灭.由热力学数据确定了两者的主要作用力类型为疏水作用力.根据F(o)rster非辐射能量转移理论,得到给体-受体间的作用距...

Interaction between ambroxol hydrochloride and human serum albumin studied by spectroscopic and molecular modeling methods

In the present paper, the interaction between ambroxol hydrochloride (ABX) and human serum albumin (HSA) was studied under simulative physiological condition by spectroscopy and molecular modeling method. Stern-Volmer curvers at different temperatures and UV-Vis absorption spectroscopy showed that ABX quenched the fluorescence of HSA mainly through dynamic quenching mode. On the basis of the thermodynamic data, the main binding force between them is hydrophobic interaction. According to the theory of Forster non-radiation energy transfer, the binding distance between the donor and the acceptor was 3.01 nm. The effect of ABX on the conformation of HSA was analyzed by the synchronous and three-dimensional fluorescence spectroscopy. Furthermore, using the molecular modeling method, the interaction between them was predicted from molecular angle: ABX might locate in the subdomain III A of HSA.