| 杜仲中活性成分分析条件的优化研究 |
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| 引用本文： | 彭密军,周春山,刘建兰,张敏,雷启福.杜仲中活性成分分析条件的优化研究[J].光谱学与光谱分析,2004,24(12):1655-1658. |
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| 作者姓名： | 彭密军 周春山 刘建兰 张敏 雷启福 |
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| 作者单位： | 吉首大学张家界校区,湖南,张家界,427000;中南大学化学化工学院,湖南,长沙,410083;中南大学化学化工学院,湖南,长沙,410083;吉首大学张家界校区,湖南,张家界,427000 |
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| 基金项目： | 湖南省科技厅攻关计划项目 (0 3NKY2 0 1 9)，湖南省教育厅青年基金 (0 2B0 2 8)资助 |
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| 摘 要： | 采用均匀设计法对对二甲氨基苯甲醛显色法测定桃叶珊瑚甙的显色条件进行了优化。建立了测定桃叶珊瑚甙的高灵敏方法 (ε=4 3× 10 4L·mol-1·cm-1) ,线性范围为 4 5～ 4 5 μg·mL-1,其灵敏度较文献报道提高 2 5倍。研究了显色剂用量、显色时间、显色温度、酸用量等因素对桃叶珊瑚甙显色反应的影响。该法应用于杜仲提取物中桃叶珊瑚甙的测定 ,结果令人满意。
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| 关 键 词： | 桃叶珊瑚甙 对二甲氨基苯甲醛 分光光度法 均匀设计法 |
| 文章编号： | 1000-0593(2004)12-1655-04 |
| 修稿时间： | 2003年9月6日 |
Study on Optimizing the Analysis Condition for Active Compound in Eucommia Olive |
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| Mi-jun Peng,Chun-shan Zhou,Jian-lan Liu,Min Zhang,Qi-fu Lei.Study on Optimizing the Analysis Condition for Active Compound in Eucommia Olive[J].Spectroscopy and Spectral Analysis,2004,24(12):1655-1658. |
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| Authors: | Mi-jun Peng Chun-shan Zhou Jian-lan Liu Min Zhang Qi-fu Lei |
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| Institution: | Jishou University, Zhangjiajie 427000, China. |
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| Abstract: | The absorption spectroscopic determination of aucubin by condensation with p-dimethylaminobenzaldehyde was optimized in this paper. A high sensitive method was established and the linearity range of the method was from 4.5 to 45 microg x mL(-1). The influences of the factors, such as the dosage of coloring agent, reaction time, heating temperature and the dose of acid etc., on the system were studied. And the result indicated that all of the factors had obvious effects on the system. The method has been applied to determining the content of aucubin in the extract of Eucommia olive with satisfactory results. |
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| Keywords: | Aucubin p-dimethylaminobenzaldehyde Absorption spectroscopy Uniform experimental design |
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