双重傅里叶变换滤波二阶比光谱导数分光光度法同时测定苯酚、邻苯二酚及对苯二酚

本文针对三组分混合体系分析提出了双重傅里叶变换滤波二阶比光谱导数分光光度法。对所测的吸收光谱用较大滤波窗口先后进行两次滤波 ;利用两次对混合体系光谱和单组分光谱比光谱求导使某两个组分对混合体系比光谱导数的贡献为零 ,而剩余组分的比光谱导数与其浓度成正比。对双重傅里叶变换滤波效果、实验参数的优化作了研究。用此方法对苯酚、邻苯二酚及对苯二酚含量为 0 0 1 0 0 9～ 0 0 5 3 2 5mg·mL- 1 的混合溶液进行测定 ,所得结果的相对误差为 0 0 7%～ 5 4%。

Simultaneous Determination of Mixed Phenol,Catechol,and Quinol by Double Fourier Transform Filtering and Second Ratio Spectra Derivative Spectrophotometry

A new spectrophotometric method for the simultaneous determination of three compounds in the same sample is proposed. The method is based on the combination of double Fourier transform filtering with second ratio spectra derivative. Each absorption spectrum is filtered twice using the bigger filter window than in the filtering once. The ratio spectrum is obtained by dividing the amplitudes of the absorption spectrum of the mixture by a standard spectrum of one of the components and getting the first derivative of the ratio spectrum of the two compounds. Then the derivative is divided by the corresponding first derivative of one of the two compounds and the second derivative is obtained. The second derivative at certain wavelength is proportional only to the concentration of one compound. The effect of double Fourier transform filtering and the optimization of experimental parameters have been studied. The method has been applied to the simultaneous determination of 0.01009-0.05325 mg.mL-1 phenol, catechol and quinol in a solution. The relative errors are between 0.07% and 5.4%.