光谱法研究杀鼠剂溴鼠灵与环糊精超分子体系及分析应用

利用光谱法研究了β-环糊精与二代抗凝杀鼠剂溴鼠灵的超分子作用,根据Benesi-Hildebrand法确定二者的包络比为1∶1,室温下包络常数为1.048×104L.mol-1。实验还考察了二者的包络机理,推测主要是溴鼠灵结构中的疏水基团联苯基进入了β-环糊精的疏水空腔。同时发现,超分子包络物的形成可以大大增强溴鼠灵的荧光发射,据此,建立了水溶液中测定溴鼠灵的荧光分析方法。在优化实验条件下,线性范围8.0×10-8~4.0×10-6mol.L-1(r=0.999 4),检出限为8.8×10-9mol.L-1,并成功地用于环境水中微量溴鼠灵的测定。

Spectrofluorimetric Study of Supramolecular Inclusion between Rodenticide Brodifacoum and β-Cyclodextrin and Application

The supramolecular interaction between β-cyclodextrin and brodifacoum, an anticoagulant rodenticide of the second generation, was studied by spectroscopy. The results showed that brodifacoum and β-cyclodextrin could form an inclusion com-plex with an association constant of 1.048×10~4 L·mol~(-1) and a 1 : 1 stoichiometry based on Benesi-Hildebrand equation. The inclusion mechanism was proposed to explain the inclusion mode. It was indicated that the hydrophohie group of brodifacoum molecule, biphenyl, entered into the cavity of β-cyclodextrin. At the same time, it was also observed the significant enhancement of fluorescence of brodifacoum after forming inclusion complex. According to the fluorescence enhancement phenomenon, a spec-trofluorimetric method of detecting brodifacoum in aqueous media was established with the linear range of 8.0×10~(-8)-4.0×10~(-6) mol·L~(-1) and the correlation coefficient of 0. 999 4. The detection limit of the method was 8.8×10~(-9) mol · L~(-1). The proposed method was successfully applied to determine the trace amount of brodifacoum in environment water and the recovery was in the range of 87.3% to 103.9%.