不同苯原酚化合物用于GOD-Trinder法测定血糖的比较研究

对苯酚、2,4-二氯苯酚、2,6-二氯苯酚及间-苯二酚等四种苯原酚化合物作为过氧化物酶(POD)催化4-氨基安替比林(4-AA) -过氧化氢-酚显色体系测定血糖的葡萄糖氧化酶法(GOD-Trinder)进行比较研究。结果表明，苯酚、2,4-二氯苯酚及间-苯二酚三种苯原酚分别构成GOD-POD-4-AA-H₂O₂-酚显色体系测定血糖时，其检测结果均无显著性差异(P＞0.05)，但2,4-二氯苯酚显色体系的显色灵敏度、方法的精密度和准确度均优于苯酚显色体系。间-苯二酚显色体系灵敏度不及苯酚，但检测速度快，线性范围宽，可用于较高浓度葡萄糖溶液的在线快速分析。

Comparative Study on GOD-Trinder Method for Measuring Glucose in Serum with Different Benzene-Original Phenols

Comparative studies were carried out on GOD-trinder method for measuring glucose in serum with four different kinds of benzene-original phenols-phenyl hydroxide, 2,4-dichlorophenol, 2,6-dichlorophenol and resorcinol. It is shown that when the three coloring systems-phenyl hydroxide, 2,4-dichlorophenol or resorcinol coupling with glucose oxidase-peroxidase-4-a minoantipyrine-hydrogen peroxide (GOD-POD-4-AA-H_2O_2) respectively were used for clinical assay of glucose in serum, the determination results showed no significant differences (P>0.05). Compared with phenyl hydroxide, the coloring system of 2,4-dichlorophenol is better in the aspects of sensitivity, precision and accuracy; the resorcinol system has lower sensitivity with the advantage of faster detecting velocity, and larger linear range, and can be used for on-line rapid analysis of samples with more concentrated glucose in industry.