基于钌配合物荧光猝灭氧二次传感BOD检测的研究

考察了利用溶胶-凝胶基质制备的氧荧光传感膜的光谱性质，实验结果表明，溶胶-凝胶基质与Ru(dpp)₃(ClO₄)₂配合物的基态之间不存在较强的相互作用，具有较好的透光性。将氧光化学传感器作为二次传感，制备了响应良好的光化学BOD微生物传感器，研究结果表明这种传感器对于较低浓度的BOD含量有着良好的线性范围，并且其响应活性保持良好，具有较长的使用寿命和良好的稳定性。

Fluorescence Characterization of Oxygen Sensing Film for BOD Determination

The film doped with tris-(4,7-diphenyl-1,10-phenanthroline) ruthenium (II) (Ru(dpp)3]2+) as an oxygen quenching indicator exhibited a good linear relationship, fast response time, long-term stability, and enhanced sensitivity to dissolved oxygen after optimizing the sol-gel processing parameters. Fiber-optical microbial sensors for the determination of biochemical oxygen demand (BOD) were described. The sensing films consist of two layers of an oxygen-sensitive fluorescent material, and three different kinds of seawater microorganisms immobilized in poly(vinyl alcohol) sol-gel matrix. The fluorescent properties and the response behaviors of the film were investigated. The effects of temperature, pH and sodium chloride concentration on the sensing films were studied as well. For low biochemical oxygen demand, the film of sieved bacteria from seawater was superior in respect of sensitivity and is expected for further development.