| 一种测定四环素类抗生素的光谱新方法 |
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| 引用本文： | 高红,赵一兵,郭祥群.一种测定四环素类抗生素的光谱新方法[J].光谱学与光谱分析,2006,26(3):488-490. |
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| 作者姓名： | 高红 赵一兵 郭祥群 |
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| 作者单位： | 厦门大学化学化工学院化学系,现代分析科学教育部重点实验室,福建,厦门,361005 |
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| 摘 要： | 四环素类抗生素属于弱荧光物质,不能在荧光计上直接检测,一般需要经过衍生反应.为了能在荧光计上实现了四环素类抗生素的直接检测,必须采用新的光谱技术,使荧光计达到了一机两用的功能.实验结果表明四环素类抗生素的线性范围均为0.10～9.00μg·mL-1,四环素、土霉素、金霉素和多西环素的检测限分别为0.065,0.067,0.068和0.070μg·mL-1.
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| 关 键 词： | 四环素 钙黄绿素 分光光度法 |
| 文章编号： | 1000-0593（2006）03-0488-03 |
| 收稿时间： | 2005-05-08 |
| 修稿时间： | 2005-08-26 |
Determination of Tetracyclines by a New Spectrum Technique |
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| GAO Hong,ZHAO Yi-bing,GUO Xiang-qun.Determination of Tetracyclines by a New Spectrum Technique[J].Spectroscopy and Spectral Analysis,2006,26(3):488-490. |
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| Authors: | GAO Hong ZHAO Yi-bing GUO Xiang-qun |
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| Institution: | Department of Chemistry and the Key Laboratory of Analytical Sciences of the Ministry of Education, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China. |
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| Abstract: | Tetracyclines are light-fluorescence substances, which cannot be detected directly by fluorometry. Herein a new spectrum method was proposed to detect tetracyclines directly by fluorometry. Under optimal conditions, the calibration graph is linear over the range 0.10-9.00 microg x mL(-1) for tetracyclines, and the detection limits of tetracycline, oxytetracycline, chlortetracycline and doxycycline are 0.065, 0.067, 0.068 and 0.070 microg x mL(-2), respectively. |
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| Keywords: | Tetracycline Calcein Spectrophotometry |
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