微波消化冷原子荧光法测定鲨鱼肝脏中的汞

研究了冷原子荧光法测定鲨鱼肝脏中汞的工作条件。样品风干后在2 mol·L-1 HNO₃-4 mol·L-1 HCl体系中以V₂O₅为催化剂，微波消解样品，上清液中的汞被SnCl₂还原后于253.7 nm(激发光)用冷原子荧光法测定，线性范围0～0.2 ng·mL-1，r=0.999 7，检出限为0.05 ng·mL-1，相对标准偏差0.86%～2.22%，平均回收率在96.0%～108.5%，该法简便、快速、准确。

Determination of Mercury in Shark Liver by Cold Atom Fluorescence Spectrometry after Microwave Dissolution

The conditions for the determination of mercury in shark liver by cold atom fluorescence spectrometry (CAFS) with microwave dissolution were studied. After being dried completely, the method employed 2 mol x L(-1) HNO3-4 mol x L(-1) HCl as an oxidant, and with catalysis by V2O5, the samples were digested in a microwave oven. The mercury in absorption solution was reduced by SnCl2, and then was determined by CAFS at wavelength of 253.7 nm. 10% SnCl2 solution was used as a reductive agent for mercury. The linear range was 0-2.0 ng x mL(-1) mercury (r = 0.999 7). The detection limit was 0.05 ng x mL(-1), the relative standard deviation was 0.86%-2.22%, and the average recovery rate was 96.0%-108.5%. The method was suitable for the determination of mercury in shark liver.