荧光探针法研究10种中药多糖及黄酮对DNA的保护作用

初步研究了金银花、灵芝等10种中草药多糖和黄酮类化合物对DNA的保护作用。利用溴化乙锭(EB)作为荧光探针，测定金银花、灵芝等10种中药多糖和黄酮类化合物存在下DNA与EB混合液的荧光积分强度，并把不同药物与DNA相互作用定义为作用常数D，D值越大，药物与DNA作用越强，反之越差。根据D的大小讨论了多糖及黄酮类化合物的存在下对DNA保护作用的影响。结果表明，10种中药均能与DNA相互作用，但作用程度不同。多糖提取物存在下它们的作用强弱顺序为：沙棘＞知母＞刺五加＞灵芝＞牛膝＞枸杞＞金银花＞苍术＞黄芪＞菊花。黄酮提取物存在下它们的作用强弱顺序为：沙棘＞金银花＞槲皮素＞枸杞＞苍术＞黄芪＞知母＞刺五加＞灵芝＞牛膝。

Study on the Role of Polysaccharide and Flavone in Chinese Herbs in Protecting DNA with Fluorescence Probe

The aim of the present work is to study the role of polyaccharide and flavone compounds of Flos lonicerae, Ganoderma lucidum etc. in protecting DNA with EB as a probe to determine the fluorescence integration intensity of DNA and EB mixture. D is defined as the interaction constant between different extracts from traditional Chinese herbs and DNA. The bigger the D, the stronger the interaction of drugs and DNA. According to the definition of D, the impact of polysaccharide and flavone compounds on the protection of DNA is discussed. The results show that all the extracts from traditional Chinese herbs can interact with DNA, but the degree of interaction is different. The order is as follows: Folium hippophae rhamnosides>Rhizoma anemarrhenae>Radix acanthopanacis senticosi>Ganoderma lucidum>Radix achyranthis bidentatae>Fructus lycii>Flos lonicerae>Rhizoma atractylodis>Astragalus membranaceus>Flos chrysanthemi in the presence of polysaccharide compounds, while Folium hippophae rhamnosides>Flos lonicerae>Fructus lycii>Rhizoma atractylodis>Astragalus membranaceus>Rhizoma anemarrhenae>Radix acanthopanacis senticosi>Ganoderma lucidum>Radix achyranthis bidentatae(Flos chrysanthemi, with no signals) in the presence of flavone compounds.