| 荧光光谱法研究肌红蛋白活性中心铁卟啉与铜离子的相互作用 |
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| 引用本文： | 冯玉英,杨辉,顾晓天,姜慧君,陆天虹.荧光光谱法研究肌红蛋白活性中心铁卟啉与铜离子的相互作用[J].光谱学与光谱分析,2003,23(3):532-534. |
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| 作者姓名： | 冯玉英 杨辉 顾晓天 姜慧君 陆天虹 |
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| 作者单位： | 1. 南京师范大学分析测试中心,材料科学重点实验室,江苏,南京,210097
2. 南京师范大学化学与环境科学学院,江苏,南京,210097 |
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| 基金项目： | 江苏省教育厅自然科学基金资助项目 (2 0 0 0LHZOOOOSJI和 0 1KJB1 50 0 0 4 )，国家自然科学基金资助项目 (2 99730 1 5) |
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| 摘 要： | 首次应用荧光光谱法研究了肌红蛋白 (FePP Mb)的活性中心铁卟啉与铜离子的相互作用 ,结果表明在水溶液中肌红蛋白中的铁可被铜离子取代 ,形成铜卟啉化合物 (CuPP Mb) ,并且随着体系中铜离子量的增加 ,其相互作用增强。本课题从蛋白质的角度研究了金属离子同生物大分子相互作用的现象并探讨了作用机理 ,可以为进一步研究有害重金属在人体内作用及毒害机理提供一定的理论依据。同时 ,本文采用的荧光光谱法与常规的UV和CD法相比有较高的灵敏度。
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| 关 键 词： | 肌红蛋白 荧光光谱 铁卟啉 |
| 文章编号： | 1000-0593(2003)03-0532-03 |
| 修稿时间： | 2002年4月15日 |
Fluorescence Spectroscopic Study of Interaction between Fe-protoporphyrin in Myoglobin and Cu(Ⅱ) Ions |
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| FENG Yu-ying,YANG Hui,GU Xiao-tian,JIANG Hui-jun and LU Tian-hong . Analysis and Testing Center,Nanjing Normal University,Nanjing ,China.Fluorescence Spectroscopic Study of Interaction between Fe-protoporphyrin in Myoglobin and Cu(Ⅱ) Ions[J].Spectroscopy and Spectral Analysis,2003,23(3):532-534. |
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| Authors: | FENG Yu-ying YANG Hui GU Xiao-tian JIANG Hui-jun and LU Tian-hong Analysis and Testing Center Nanjing Normal University Nanjing China |
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| Institution: | Analysis and Testing Center, Nanjing Normal University, Nanjing 210097, China. |
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| Abstract: | In this paper, the interaction between Cu(II) ions and Fe-protoporphyrin in horse-heart myoglobin (FePP-Mb) was studied. As a result, some of the Fe(II) ions in FePP-Mb were found to be replaced by Cu(II) ions forming CuPP-Mb, by adding Cu(II) ions into the myoglobin solution. The interaction became stronger when adding more Cu(II) ions into the myoglobin solution. By studying the metal ions' interaction with myoglobin proteins as macromolecules and discussing the interaction mechanism, this work provides a theoretical basis for the further study of hazardous metal ions' interaction with the human body and its mechanism. The fluorescence spectroscopic method used in this study has higher sensitivity than the ordinary UV and CD methods. |
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| Keywords: | Myoglobin Fluorescence Fe-protoporphyrin |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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