| 甲苯胺蓝共振光散射法测定纳克级脱氧核糖核酸 |
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| 引用本文： | 肖锡林,王永生,李贵荣,吕昌银.甲苯胺蓝共振光散射法测定纳克级脱氧核糖核酸[J].光谱学与光谱分析,2004,24(2):190-193. |
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| 作者姓名： | 肖锡林 王永生 李贵荣 吕昌银 |
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| 作者单位： | 南华大学公共卫生学院,湖南,衡阳,421001;南华大学公共卫生学院,湖南,衡阳,421001;南华大学公共卫生学院,湖南,衡阳,421001;南华大学公共卫生学院,湖南,衡阳,421001 |
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| 基金项目： | 湖南省教育厅科研基金(01C213)资助项目 |
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| 摘 要： | 研究了吩噻嗪类染料甲苯胺蓝 (TB)与DNA作用的共振光散射光谱 ,在pH 1 0～ 1 1的范围内 ,加入DNA导致甲苯胺蓝共振光散射增强 ,在 35 0nm处 ,存在一共振光散射增强峰 ,其强度与DNA浓度呈线性关系 ,据此建立了一种测定DNA的共振光散射法。对于ctDNA ,方法的线性范围为 0～ 90 0ng·mL-1 ,检出限为6 75ng·mL-1 ,RSD为 3 7% ;对于fsDNA ,方法的线性范围为 0～ 90 0ng·mL-1 ,检出限为 2 99ng·mL-1 ,RSD为 5 6 % .已用于合成样品中DNA的测定
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| 关 键 词： | 甲苯胺蓝 共振光散射法 脱氧核糖核酸 |
| 文章编号： | 1000-0593(2004)02-0190-04 |
| 修稿时间： | 2003年1月13日 |
Determination of Deoxyribonucleic Acids at Nanograms Levels with Toluidine Blue by a Resonance Light-Scattering Method |
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| Xi-lin Xiao,Yong-sheng Wang,Gui-rong Li,Chang-yin Lü.Determination of Deoxyribonucleic Acids at Nanograms Levels with Toluidine Blue by a Resonance Light-Scattering Method[J].Spectroscopy and Spectral Analysis,2004,24(2):190-193. |
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| Authors: | Xi-lin Xiao Yong-sheng Wang Gui-rong Li Chang-yin Lü |
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| Institution: | College of Public Health, Nanhua University, Hengyang 421001, China. |
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| Abstract: | Resonance light scattering (RLS) spectra of toluidine blue (TB) with DNA was studied. The RLS of TB was greatly enhanced by DNA in the range of pH 10-11. A RLS peak at 350 nm was found, and the enhanced intensity of RLS at this wavelength was proportional to the concentration of DNA. The linear range of the calibration curve was 0-900 ng x mL(-1) with the detection limit of 6.75 ng x mL(-1) for the ctDNA, and for fsDNA the linear range was 0-900 ng x mL(-1) with the detection limit of 2.99 ng x mL(-1). Precision at 500 ng x mL(-1) for the two nucleic acids was 3.7% and 5.6%, respectively. Four synthetic samples were determined satisfactorily. |
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| Keywords: | Toluidine blue Resonance light scattering Calf thymus DNA Fish sperm DNA |
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