蛇足石杉及其近缘种表征关系的FTIR主成分分析

蛇足石杉是一种重要药用植物，其种内变异程度高，近缘种多，植株体型小，可供鉴别性状少。运用OMNI采样器-傅里叶变换红外光谱直接测定法，获得了蛇足石杉(Huperzia Serrata)及其5个近缘种：四川石杉(Huperzia Sutchueniana)、闽浙马尾杉(Phlegmariurus Mingchegensis)、石松(Lycopodium Japonicum)、深绿卷柏(Selaginella Doederleinii)和异穗卷柏(Selaginella Heterostachys)16个叶片样品的红外光谱，以666～3 145 cm-1范围内吸收峰吸光度为指标，以红外光谱图为对象，应用主成分分析(Principal Component Analysis)，比较了16个样品在红外光谱上的差异程度。发现基于傅里叶变换红外光谱的主成分分析排序图能够在一定程度上表征16个样品的分类关系。应用FTIR直接测定法能够鉴别蛇足石杉及其近缘物种。

FTIR Spectra-Principal Component Analysis of Phenetic Relationships of Huperzia Serrata and Its Closely Related Species

Huperzia serrata is an important medicinal plant. This species is rich in inner-specific variation with various closely related species, and their individuals are also small with few identification characters. In the present paper, the method of Fourier transform infrared spectrometer with an OMNI collector was applied to obtaining the infrared spectra of 16 leaf samples including Huperzia serrata and its five closely related species (Huperzia sutchueniana, Phlegmariurus mingchegensis, Lycopodium japonicum, Selaginella doederleinii, Selaginella heterostachys). Based on the indices of wave number-absorbance, the differences of the 16 infrared spectra were compared by the method of Principal Component Analysis (PCA). The results showed that there is good correspondence between the position relationship of PCA three-dimensional plot of the samples based on the indices of wave number-absorbance of FTIR spectra and their phenetic relationship. Therefore, the infrared spectra could be applied to identifying the samples of Huperzia serrata and its closely related species.