| EB荧光探针法研究多粘菌素B与DNA的作用方式 |
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| 引用本文： | 江崇球,贺吉香,王金山.EB荧光探针法研究多粘菌素B与DNA的作用方式[J].光谱学与光谱分析,2002,22(1):103-106. |
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| 作者姓名： | 江崇球 贺吉香 王金山 |
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| 作者单位： | 1. 山东师范大学化学系,山东,济南,250014
2. 山东省中医药大学中药学院,山东,济南,250014 |
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| 基金项目： | 山东省自然科学基金资助项目 |
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| 摘 要： | 本文以溴化乙锭（EB）为荧光探针，对多粘菌素B（Polymyxin B sulfate)与DNA的作用机制进行了研究。对荧光光谱法、紫外光谱法、荧光偏振法、热变性等方面的研究结果表明：在pH=7.4的溶液中PB与DNA双螺旋碱基对之间存在嵌插作用，在一定离子强度下该嵌插作用会减弱。此外，还存在着非特性的静电作用。
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| 关 键 词： | 荧光光谱 多粘菌素B 脱氧核糖核酸 嵌插剂 溴化乙锭 DNA EB 荧光探针 多肽类抗生素 药物作用机理 |
| 文章编号： | 1000-0593(2002)01-0103-04 |
| 修稿时间： | 2000年12月8日 |
Studies on the Interaction Mode between Polymyxin B Sulfate and DNA with EB as a Fluorescence Probe |
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| JIANG Chong-qiu ,HE Ji-xiang ,WANG Jin-shan .Shandong Normal University,Jinan ,China .Shandong University of Traditional Chinese Medicine,Jinan ,China.Studies on the Interaction Mode between Polymyxin B Sulfate and DNA with EB as a Fluorescence Probe[J].Spectroscopy and Spectral Analysis,2002,22(1):103-106. |
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| Authors: | JIANG Chong-qiu HE Ji-xiang WANG Jin-shan Shandong Normal University Jinan China Shandong University of Traditional Chinese Medicine Jinan China |
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| Institution: | Shandong Normal University, Jinan 250014, China. |
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| Abstract: | The interaction between polymyxin B sulfate and ct-DNA was studied with ethidium bromide (EB) probe by UV spectroscopy,fluorescence spectroscopy, fluorescent polarization etc.The phenomena of hyperchromism effect and fluorescence quenching proved that polymyxin B sulfate in buffer solution at pH 7.4 can intercalate into the base pairs of ct-DNA.This interaction can cause conformational change of ct-DNA double helix.This interaction can be inhibited in the presence of high concentration of ions.At the same time,PB can combine with the phosphate group of ct-DNA by non-characteristic static force.In this paper,the interaction of PB and ct-DNA was made further discussion. |
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| Keywords: | Fluorescence spectrum Polymyxin B sulfate Deoxyribonucleotide Intercalation |
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