氯氧化锆-氯化铵荧光光谱法测定葡萄糖共存下的果糖

文章根据ZrOCl₂水解产生的ZrO(OH)]+能与果糖作用产生荧光的原理，建立了果糖的定量测定方法。在NH₄Cl介质中，体系的荧光强度有所增强，产物在408 nm处发荧光，在0.5～25 μg·mL-1范围内荧光强度与果糖浓度成正比，当反应温度为70 ℃、反应时间15 min时，葡萄糖不干扰测定。该法成功用于水果中果糖的测定，灵敏度高，检出限达0.071 μg·mL-1,方法稳定性好，结果满意。

Fluorometric Determination of Fructose in the Presence of Glucose Using Zirconyl Chloride-Ammonium Chloride

According to the principle that Zn(OH)+ produced by ZrOCl2 upon hydrolysis in water can react to form a fluorescent derivative with fructose, a method for the quantitative determination of fructose has been proposed. The fluorescence of the derivative enhanced at 408 nm in NH4Cl medium has a direct ratio with fructose in the linear range of 0.5-25 microg x mL(-1). When reaction conditions such as a temperature of 70 degrees C and a time of 15 min are used, the detection limits is 0.071 mg x mL(-1). The proposed method was also applied successfully to the determination of fruit, satisfaction with high accuracy and good reproduction.