流动注射化学发光法对安乃近进行选择性定量测定

通过对药物中安乃近的选择性定量测定 ,提出一种新的化学发光分析法。当安乃近溶液与酸性的聚乙二醇 4 0 0及罗丹明 6G混合溶液相遇时 ,会产生强烈的化学发光信号 ,根据光信号的大小即可定量测定安乃近的含量。反应中无任何氧化剂使用 ,因此 ,很难解释光信号产生的原因 ,该反应发光机理仍在探讨之中。这种测定方法具有极高的选择性 ,无论被测物是否含有磺酸基团 ,实验中未发现任何其他药物或化学物质会产生有意义的光信号 ,所以 ,不会象其他化学发光体系存在其他物质干扰问题。另外 ,此反应具有高度的灵敏性 ,其工作曲线线性范围为 0 0 1～ 1 0 0 μg·mL-1 ,检出限为 0 0 0 3μg·mL-1 ,此为目前安乃近的所有化学发光测定法中最低的检出限值。该测定方法已成功用于针剂及片剂药物中乃安近的定量测定

Selective Quantitation of Analgin by Flow Injection Chemiluminescence Method

A new chemiluminescence-based method was proposed for selective quantitation of analgin in pharmaceutical preparation. The CL emission was generated by mixing analgin, polyethylene glycol-400 and rhodamine 6G in acidic medium. The analgin content could be determined by the CL intensity. No oxidants were used in the system, so it was difficult to explain why the CL emission was produced. The mechanism of CL emission remains under investigation. One of the advantages of this method is highly selective. No other pharmaceutical compounds and chemicals could produce CL emission whether they contain sulfuric group or not in the experiment. There was no disturbance in this method, which was different from others. Another advantage is high sensitivity. The quantitation range is from 0.01 to 10 microg x mL(-1) with a detection limit of 0.003 microg x mL(-1) for analgin. This is the lowest detection limit of any CL methods currently available for analgin determination. The proposed method was applied to the selective quantitation of analgin in pharmaceutical preparation.