十二烷基硫酸钠敏化联苯胺共振光散射技术测定脱氧核糖核酸

研究了在十二烷基硫酸钠作用下联苯胺与小牛胸腺脱氧核糖核酸 (ctDNA)作用的共振光散射光谱(RLS)的特征。痕量核酸对联苯胺 十二烷基硫酸钠的RLS有增强作用 ,且增强的程度与核酸的浓度成线性关系。在优化条件下确定了RLS强度与核酸浓度的线性范围为 0 3～ 4mg·L-1,线性方程为I =37 17c(ctDNA ,mg·L-1) +2 0 8 9,相关系数r =0 9992。方法的检出限为 0 17mg·L-1(3δ) ,相对标准偏差3 5 %。该方法成功地用于人工混合样品和转基因烟草中的DNA含量测定

Determination of Deoxyribonuceic Acids by Resonance Light Scattering Technique with Benzidine in the Presence of the Sensitizing Sodium Dodecyl Sulfate

The resonance light scattering (RLS) spectra of benzidine with deoxyribonucleic acid (ctDNA) in the presence of Sodium Dodecyl Sulfate had been studied. RLS intensity of SDS-benzidine system was enhanced by trace ctDNA and was proportional to the concentrationof ctDNA. Under optima conditions, the linear ranges of the calibration were 0.3-4 mg x L(-1), the linear regression equation was I = 37.17c (ctDNA, mg x L(-1)) + 208.9, r = 0.9992. The limit of detection were 0.17 mg x L(-1) (3delta), RSD% = 3.5%. The method had been applied to the determination of DNA in synthetic samples and gene tobacco sample, and the results were satisfactory.