| 中性红共振光散射法测定脱氧核糖核酸 |
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| 引用本文： | 向海艳,陈小明,李松青,夏殊,刘安喜.中性红共振光散射法测定脱氧核糖核酸[J].光谱学与光谱分析,2001,21(6):822-825. |
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| 作者姓名： | 向海艳 陈小明 李松青 夏殊 刘安喜 |
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| 作者单位： | 湘潭大学化学化工学院,湖南,湘潭,411105 |
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| 基金项目： | 湖南省自然科学基金资助项目 (资助号 0 0JJY2 0 84) |
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| 摘 要： | 本文基于脱氧核糖核酸 (DNA)对有机染料中性红的共振光散射的增强效应 ,拟订了一种测定DNA的共振光散射法。在pH 5 0～ 7 0范围内 ,有机染料中性红在DNA分子表面进行长距组装导致共振光散射增强。在 335 0nm处的共振光谱散射增强程度与DNA浓度呈线性关系 ,其线性范围为 0～ 6 0 0ng·mL-1,检出限可达 12 8ng·mL-1。该方法简便、快速 ,具有较高的灵敏度和准确度。将该方法用于混合样品中DNA的测定 ,结果令人满意。
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| 关 键 词： | 中性红 脱氧核糖核酸 DNA 测定 共振光散射法 混合样品分析 |
| 文章编号： | 1000-0593(2001)06-0822-04 |
| 修稿时间： | 2000年5月29日 |
Determination of Deoxyribonucleic Acid with Neutral Red by Resonance Light Scattering Method |
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| XIANG Hai yan,CHEN Xiao ming,LI Song qing,XIA Shu,and LIU An xi Institute of Chemistry and Chemical Engineering,Xiangtan University,Xiangtan ,China.Determination of Deoxyribonucleic Acid with Neutral Red by Resonance Light Scattering Method[J].Spectroscopy and Spectral Analysis,2001,21(6):822-825. |
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| Authors: | XIANG Hai yan CHEN Xiao ming LI Song qing XIA Shu and LIU An xi Institute of Chemistry and Chemical Engineering Xiangtan University Xiangtan China |
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| Institution: | Institute of Chemistry and Chemical Engineering, Xiangtan University, Xiangtan 411105, China. |
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| Abstract: | The method is based on the enhancement effect of the resonance light scattering (RLS) due to the long range assembly of neutral red (NR) on the molecular surface of DNA. The RLS of NR is greatly enhanced by DNA in the pH range of 5.0-7.0. The enhanced intensity of RLS at 335.0 nm is proportional to the concentration of DNA in the range 0-600 ng.mL-1. The detection limit is 12.8 ng.mL-1. This method is simple, rapid and has been applied to the determination of DNA in mixed samples with satisfactory results. |
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| Keywords: | Neutral red DNA Resonance light scattering method |
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