应用红外光谱研究电场对超氧化物歧化酶二级结构的影响

超氧化物歧化酶经不同强度的电场处理，利用红外光谱法研究电场对超氧化物歧化酶二级结构的影响。结果表明，经不同电场强度处理的超氧化物歧化酶(SOD)，其二级结构单元百分含量有不同程度的变化。α-螺旋和β-折叠结构单元在电场处理后，均为减少;β-转角结构单元均为增加;无规卷曲结构单元除4.0 kV·cm-1电场处理条件减少外，其余电场处理条件均为增加。电场处理具有使超氧化物歧化酶的α-螺旋、β-折叠向β-转角和无轨卷曲结构转化的作用，且不同电场条件下其转化情况不同。研究结果还表明，电场的作用使SOD活性发生改变，SOD活性变化与β-折叠含量变化相关。

Study on the Effect of Electric Field on the Secondary Structure of SOD by FTIR Spectroscopy

After the SOD (superoxide dismutase) was treated with different strength of electric field, the effect of electric field on the secondary structure of SOD was studied by FTIR (Fourier-transformation infrared spectroscopy). The results have shown that different electric field strength has different effects on the secondary structure contents of the SOD. As compared with the contrast, the relative contents of alpha-helix and beta-sheet were decreased, while beta-turn content increased. The random coil contents were increased except for the 1.0 kV x cm(-1). The electric treatment tends to transform the alpha-helix and beta-sheet into beta-turn and random coil, and different strength of electric field has a different effect on the transformation. The changes in SOD activity have a direct relationship to the contents of beta-sheet.