基于回音壁谐振模的无标记光学生物传感技术

光学生物传感器在新药研制和生命科学等领域得到广泛关注,重点对基于回音壁谐振模的无标记光学生物传感器做了评述。根据谐振腔结构将传感器分为三类。基于微球的生物传感器由于微球腔的高品质因子而成为最初研究的重点,已实验研究了对蛋白质分子、病毒和细菌的传感响应,建立了基于单光子谐振能量和微扰理论的理论模型;基于微盘的生物传感器能够利用成熟的平面光刻微加工技术,传感构想提出更早,但直到回流热处理技术的应用才使得微盘品质因子大幅提高,从而实现了单分子测量;基于微环的生物传感器具有简单的谐振模式,有利于信号探测,已采用聚合物,氮化硅,以及硅基二氧化硅等材料制作成功,作为其在三维上的扩展,微管式传感器由于能够将光通道和流体通道合二为一而在近年得到关注。

Review on label-free optical bio-sensing technology based on whisper-gallery-mode

Optical biosensors are becoming an important tool for drug research and life science, and the label-free optical biosensor based on whisper-gallery-mode (WGM) is reviewed in the present paper. The WGM-based sensors are categorized into three types according to the microcavity structure. The biosensor using microsphere got extensive research because of high quality factor, and its response to protein, virus, and bacteria had been studied. The models based on single photon resonant state and perturbation theory were established. The biosensor using microdisk was proposed early since it can make use of mature lithography technology; however, the quality factor was increased greatly only after the thermal reflow process was introduced and single molecule measurement was then realized. The biosensor using microring has simpler mode structure and materials such as polymer, silicon nitride and silicon-on-insulator had been used for sensor fabrication. As a 3-dimension expansion, sensor using microtube can combine the optical channel and fluidic channel, which attracting more and more attention.