| 近红外光谱法测定双黄连口服液中绿原酸和连翘苷的含量 |
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| 引用本文： | 戴传云,高晓燕,汤波,傅亚,刘火安.近红外光谱法测定双黄连口服液中绿原酸和连翘苷的含量[J].光谱学与光谱分析,2010,30(2). |
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| 作者姓名： | 戴传云 高晓燕 汤波 傅亚 刘火安 |
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| 作者单位： | 1. 重庆科技学院生物系,重庆,401331
2. 北京中医药大学科技发展中心,北京,100029 |
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| 基金项目： | 国家自然科学基金项目(30701055)资助 |
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| 摘 要： | 选择多批双黄连口服液样本,应用HPLC法测定绿原酸和连翘苷的含量,同时进行NIRS测定,建立了绿原酸和连翘苷的含量预测模型,以相对偏差(RSEP)及相关系数(r)为指标考察数据处理方法,以交互验证均方根误差(RMSECV)为指标考察NIRS的预处理方法及选择光谱范围或波数点。数据处理方法为逐步多元线性回归(SMLR),直接采用原始光谱进行建模,绿原酸的波数为6 654.06和7 106.08 cm-1,连翘苷的波数为5 456.06和7 222.08 cm-1,最优模型预测绿原酸和连翘苷含量的RMSECV分别为0.857 26和0.889 87,相关系数分别为0.857 26和0.889 87。外部交互验证的结果表明该预测模型准确可靠,可作为双黄连口服液的快速质量评价控制方法。
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| 关 键 词： | 近红外光谱（NIRS） 双黄连口服液 绿原酸 连翘苷 |
Determination of the Contents of Chlorogenic Acid and Phillyrin of Shuanghuanglian Oral Fluid Using NIRS |
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| DAI Chuan-yun,GAO Xiao-yan,TANG Bo,FU Ya,LIU Huo-an.Determination of the Contents of Chlorogenic Acid and Phillyrin of Shuanghuanglian Oral Fluid Using NIRS[J].Spectroscopy and Spectral Analysis,2010,30(2). |
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| Authors: | DAI Chuan-yun GAO Xiao-yan TANG Bo FU Ya LIU Huo-an |
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| Institution: | DAI Chuan-yun1,GAO Xiao-yan2,TANG Bo1,FU Ya1,LIU Huo-an11.Department of Biology,Chongqing University of Science , Technology,Chongqing 401331,China2.Science Development Center for Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China |
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| Abstract: | The aim of the present study was to establish the model of predicting the contents of chlorogenic acid and phillyrin in Shuanghuanglian oral fluid using NIR to realize quick quality evaluation of Shuanghuanglian oral fluid.To this end,many batches of Shuanghuanglian oral fluid were selected,and the contents of chlorogenic acid and phillyrin were determined using HPLC.Meanwhile,the NIR spectra of the same samples were determined.The model used to predict the contents of chlorogenic acid and phillyrin in Shua... |
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| Keywords: | NIRS Shuanghuanglian oral fluid Chlorogenic Phillyrin |
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